| Title |
Palmitate-induced Slc2a4/GLUT4 downregulation in L6 muscle cells: evidence of inflammatory and endoplasmic reticulum stress involvement
|
|---|---|
| Published in |
Lipids in Health and Disease, April 2018
|
| DOI | 10.1186/s12944-018-0714-8 |
| Pubmed ID | |
| Authors |
Patrícia Ebersbach-Silva, Ana Cláudia Poletto, Aline David-Silva, Patrícia Monteiro Seraphim, Gabriel Forato Anhê, Marisa Passarelli, Daniela Tomie Furuya, Ubiratan Fabres Machado |
| Abstract |
Obesity is strongly associated to insulin resistance, inflammation, and elevated plasma free fatty acids, but the mechanisms behind this association are not fully comprehended. Evidences suggest that endoplasmic reticulum (ER) stress may play a role in this complex pathophysiology. The aim of the present study was to investigate the involvement of inflammation and ER stress in the modulation of glucose transporter GLUT4, encoded by Slc2a4 gene, in L6 skeletal muscle cells. L6 cells were acutely (2 h) and chronically (6 and 12 h) exposed to palmitate, and the expression of several proteins involved in insulin resistance, ER stress and inflammation were analyzed. Chronic and acute palmitate exposure significantly reduced GLUT4 protein (~ 39%, P < 0.01) and its mRNA (18%, P < 0.01) expression. Only acute palmitate treatment increased GRP78 (28%, P < 0.05), PERK (98%, P < 0.01), eIF-2A (35%, P < 0.01), IRE1a (60%, P < 0.05) and TRAF2 (23%, P < 0.05) protein content, and PERK phosphorylation (106%, P < 0.001), but did not elicit eIF-2A, IKK phosphorylation or increased XBP1 nuclear content. Additionally, acute and chronic palmitate increased NFKB p65 nuclear content (~ 30%, P < 0.05) and NFKB binding activity to Slc2a4 gene promoter (~ 45%, P < 0.05). Different pathways are activated in acute and chronic palmitate induced-repression of Slc2a4/GLUT4 expression. This regulation involves activation of initial component of ER stress, such as the formation of a IRE1a-TRAF2-IKK complex, and converges to NFKB-induced repression of Slc2a4/GLUT4. These results link ER stress, inflammation and insulin resistance in L6 cells. |
X Demographics
The data shown below were collected from the profiles of 15 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 7 | 47% |
| United Kingdom | 3 | 20% |
| Chile | 1 | 7% |
| Australia | 1 | 7% |
| Norway | 1 | 7% |
| Unknown | 2 | 13% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 9 | 60% |
| Members of the public | 6 | 40% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 7 | 26% |
| Student > Ph. D. Student | 3 | 11% |
| Unspecified | 1 | 4% |
| Student > Doctoral Student | 1 | 4% |
| Lecturer | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 12 | 44% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 19% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 7% |
| Medicine and Dentistry | 2 | 7% |
| Agricultural and Biological Sciences | 2 | 7% |
| Unspecified | 1 | 4% |
| Other | 4 | 15% |
| Unknown | 11 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 April 2018.
All research outputs
#3,939,900
of 23,041,514 outputs
Outputs from Lipids in Health and Disease
#264
of 1,459 outputs
Outputs of similar age
#77,651
of 328,966 outputs
Outputs of similar age from Lipids in Health and Disease
#7
of 40 outputs
Altmetric has tracked 23,041,514 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,459 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.1. This one has done well, scoring higher than 81% of its peers.
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We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.