| Title |
Identification of three Zwittermicin A biosynthesis-related genes from Bacillus thuringiensis subsp. kurstaki strain YBT-1520
|
|---|---|
| Published in |
Archives of Microbiology, January 2007
|
| DOI | 10.1007/s00203-006-0196-3 |
| Pubmed ID | |
| Authors |
Changming Zhao, Yi Luo, Chunxu Song, Zhongxin Liu, Shouwen Chen, Ziniu Yu, Ming Sun |
| Abstract |
Zwittermicin A (ZwA) is a novel, broad-spectrum linear aminopolyol antibiotic produced by some Bacillus cereus and Bacillus thuringiensis. However, only part of its biosynthesis cluster has been identified and characterized from B. cereus UW85. To better understand the biosynthesis cluster of ZwA, a bacterial artificial chromosome (BAC) library of B. thuringiensis subsp. kurstaki strain YBT-1520, a ZwA-producing strain, was constructed. Two BAC clones, 1F8 and 5E2, were obtained by PCR, which overlap the known ZwA biosynthesis cluster of B. cereus UW85. This ZwA biosynthesis cluster is at least 38.6 kb and is located on the chromosome, instead of the plasmid. Partial DNA sequencing revealed both BAC clones carry three new ZwA biosynthesis-related genes, zwa6, zwa5A and zwa5B, which were found at the corresponding location of B. cereus UW85. Putative amino acid sequences of these genes shown that ZWA6 is homologous to a typical carbamoyltransferase from Streptomyces avermitilis, while ZWA5A and ZWA5B are homologs of cysteine synthetase and ornithine cyclodeaminase which jointly synthesize 2,3-diaminopropionate in the viomycin biosynthesis pathway, respectively. The identification of these three genes further supports the hypothesized ZwA biosynthesis pathway. |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Unknown | 38 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 26% |
| Student > Ph. D. Student | 7 | 18% |
| Student > Master | 5 | 13% |
| Student > Bachelor | 2 | 5% |
| Professor > Associate Professor | 2 | 5% |
| Other | 5 | 13% |
| Unknown | 8 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 46% |
| Biochemistry, Genetics and Molecular Biology | 6 | 15% |
| Immunology and Microbiology | 2 | 5% |
| Business, Management and Accounting | 1 | 3% |
| Environmental Science | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 9 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 November 2021.
All research outputs
#4,696,673
of 22,789,076 outputs
Outputs from Archives of Microbiology
#219
of 2,770 outputs
Outputs of similar age
#21,776
of 159,827 outputs
Outputs of similar age from Archives of Microbiology
#2
of 8 outputs
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So far Altmetric has tracked 2,770 research outputs from this source. They receive a mean Attention Score of 3.7. This one has done well, scoring higher than 84% of its peers.
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