| Title |
Specific and robust ion chromatographic determination of hypothiocyanite in saliva samples
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, March 2018
|
| DOI | 10.1007/s00216-018-0954-5 |
| Pubmed ID | |
| Authors |
Harald Below, Romy Baguhl, Wiebke Geßner, Axel Kramer, Elke Below, Heike Kahlert, Alexander Welk |
| Abstract |
The enzymatic system in saliva, consisting of salivary peroxidase (SPO), hydrogen peroxide (H2O2), and thiocyanate (SCN-), produces hypothiocyanite (OSCN-) as a high effective antibacterial compound. OSCN- is of great importance for the natural non-specific antibacterial resistance in the oral cavity. However, no analytical method currently exists to selectively quantify OSCN- in saliva samples. A robust and specific analytical method for the determination of OSCN- was developed based on ion chromatography with combined UV and electrochemical detection. Calibration was achieved by calculating a derived calibration factor based on the known ratio of molar extinction coefficients of SCN- and OSCN-. Thus, the specific quantification of OSCN- in saliva samples is possible, as demonstrated here. The median value of 200 saliva samples was determined to be 0.56 mg L-1 (median), with a maximum of 3.9 mg L-1; the minimum value was below the detection limit (< 0.09 mg L-1). The recovery rate in individual saliva samples was 95 ± 8%. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 30% |
| Professor > Associate Professor | 2 | 20% |
| Student > Bachelor | 1 | 10% |
| Student > Ph. D. Student | 1 | 10% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 4 | 40% |
| Agricultural and Biological Sciences | 2 | 20% |
| Chemistry | 1 | 10% |
| Unknown | 3 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 April 2018.
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#22,767,715
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Outputs of similar age from Analytical & Bioanalytical Chemistry
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