| Title |
Characterization of a novel sugar transporter involved in sugarcane bagasse degradation in Trichoderma reesei
|
|---|---|
| Published in |
Biotechnology for Biofuels and Bioproducts, April 2018
|
| DOI | 10.1186/s13068-018-1084-1 |
| Pubmed ID | |
| Authors |
Karoline M. V. Nogueira, Renato Graciano de Paula, Amanda Cristina Campos Antoniêto, Thaila F. dos Reis, Cláudia Batista Carraro, Alinne Costa Silva, Fausto Almeida, Carem Gledes Vargas Rechia, Gustavo H. Goldman, Roberto N. Silva |
| Abstract |
Trichoderma reesei is a saprophytic fungus implicated in the degradation of polysaccharides present in the cell wall of plants.T. reeseihas been recognized as the most important industrial fungus that secretes and produces cellulase enzymes that are employed in the production of second generation bioethanol. A few of the molecular mechanisms involved in the process of biomass deconstruction byT. reesei; in particular, the effect of sugar transporters and induction of xylanases and cellulases expression are yet to be known. In our study, we characterized a novel sugar transporter, which was previously identified by our group through in silico analysis of RNA-seq data. The novelT. reesei69957-sugar transport system (Tr69957) is capable of transporting xylose, mannose, and cellobiose using aT. reesei69957-sugar transport system inSaccharomyces cerevisiae. The deletion ofTr69957inT. reeseiaffected the fungal growth and biomass accumulation, and the sugar uptake in the presence of mannose, cellobiose, and xylose. Molecular docking studies revealed thatTr69957shows reduced protein-ligand binding energy for interactions towards disaccharides in comparison with monosaccharides. Furthermore, the deletion ofTr69957affected the gene expression of cellobiohydrolases (cel7aandcel6a), β-glucosidases (cel3aandcel1a), and xylanases (xyn1andxyn2) in the cultures of parental and mutant strains in the presence of cellobiose and sugarcane bagasse (SCB). The transporterTr69957ofT. reeseican transport cellobiose, xylose, and mannose, and can affect the expression of a few genes encoding enzymes, such as cellulases and xylanases, in the presence of SCB. We showed for the first time that a filamentous fungus (T. reesei) contains a potential mannose transporter that may be involved in the degradation of cellulose. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 77 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 77 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 21% |
| Student > Master | 11 | 14% |
| Researcher | 8 | 10% |
| Student > Bachelor | 7 | 9% |
| Student > Doctoral Student | 5 | 6% |
| Other | 10 | 13% |
| Unknown | 20 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 26 | 34% |
| Agricultural and Biological Sciences | 15 | 19% |
| Chemical Engineering | 3 | 4% |
| Engineering | 2 | 3% |
| Chemistry | 2 | 3% |
| Other | 5 | 6% |
| Unknown | 24 | 31% |
Attention Score in Context
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#22,767,715
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Outputs of similar age from Biotechnology for Biofuels and Bioproducts
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