| Title |
Platelet Proteomic Analysis Revealed Differential Pattern of Cytoskeletal- and Immune-Related Proteins at Early Stages of Alzheimer’s Disease
|
|---|---|
| Published in |
Molecular Neurobiology, March 2018
|
| DOI | 10.1007/s12035-018-1039-3 |
| Pubmed ID | |
| Authors |
Marta González-Sánchez, Teresa Díaz, Consuelo Pascual, Desiree Antequera, Alejandro Herrero-San Martín, Sara Llamas-Velasco, Alberto Villarejo-Galende, Fernando Bartolome, Eva Carro |
| Abstract |
Platelets are considered a good model system to study a number of elements associated with neuronal pathways as they share biochemical similarities. Platelets represent the major source of amyloid-β (Aβ) in blood contributing to the Aβ accumulation in the brain parenchyma and vasculature. Peripheral blood platelet alterations including cytoskeletal abnormalities, abnormal cytoplasmic calcium fluxes or increased oxidative stress levels have been related to Alzheimer's disease (AD) pathology. Therefore, platelets can be considered a peripheral model to study metabolic mechanisms occurring in AD. To investigate peripheral molecular alterations, we examined platelet protein expression in a cohort of 164 subjects, including mild cognitive impairment (MCI), and AD patients, and healthy aged-matched controls. A two-dimensional difference gel electrophoresis (2D-DIGE) discovery phase revealed significant differences between patients and controls in five proteins: talin, vinculin, moesin, complement C3b and Rho GDP, which are known to be involved in cytoskeletal regulation including focal adhesions, inflammation and immune functions. Western blot analysis verified that talin was found to be increased in mild and moderate AD groups versus control, while the other three were found to be decreased. We also analysed amyloid precursor protein (APP), amyloid-β 1-40 (Aβ40) and 1-42 (Aβ42) levels in platelets from the same groups of subjects. Upregulation of platelet APP and Aβ peptides was found in AD patients compared to controls. These findings complement and expand previous reports concerning the morphological and functional alterations in AD platelets, and provide more insights into possible mechanisms that participate in the multifactorial and systemic damage in AD. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 36 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 11% |
| Student > Bachelor | 4 | 11% |
| Student > Ph. D. Student | 3 | 8% |
| Professor > Associate Professor | 3 | 8% |
| Student > Master | 3 | 8% |
| Other | 7 | 19% |
| Unknown | 12 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 8 | 22% |
| Medicine and Dentistry | 7 | 19% |
| Biochemistry, Genetics and Molecular Biology | 4 | 11% |
| Psychology | 1 | 3% |
| Linguistics | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 14 | 39% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2019.
All research outputs
#3,274,073
of 23,041,514 outputs
Outputs from Molecular Neurobiology
#672
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Outputs of similar age
#68,673
of 329,487 outputs
Outputs of similar age from Molecular Neurobiology
#20
of 120 outputs
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