| Title |
Differential activity of methylene blue against erythrocytic and hepatic stages of Plasmodium
|
|---|---|
| Published in |
Malaria Journal, April 2018
|
| DOI | 10.1186/s12936-018-2300-y |
| Pubmed ID | |
| Authors |
Henriette Bosson-Vanga, Jean-François Franetich, Valérie Soulard, Daniel Sossau, Maurel Tefit, Bocar Kane, Jean-Christophe Vaillant, Steffen Borrmann, Olaf Müller, Nathalie Dereuddre-Bosquet, Roger Le Grand, Olivier Silvie, Dominique Mazier |
| Abstract |
In the context of malaria elimination/eradication, drugs that are effective against the different developmental stages of the parasite are highly desirable. The oldest synthetic anti-malarial drug, the thiazine dye methylene blue (MB), is known for its activity against Plasmodium blood stages, including gametocytes. The aim of the present study was to investigate a possible effect of MB against malaria parasite liver stages. MB activity was investigated using both in vitro and in vivo models. In vitro assays consisted of testing MB activity on Plasmodium falciparum, Plasmodium cynomolgi and Plasmodium yoelii parasites in human, simian or murine primary hepatocytes, respectively. MB in vivo activity was evaluated using intravital imaging in BALB/c mice infected with a transgenic bioluminescent P. yoelii parasite line. The transmission-blocking activity of MB was also addressed using mosquitoes fed on MB-treated mice. MB shows no activity on Plasmodium liver stages, including hypnozoites, in vitro in primary hepatocytes. In BALB/c mice, MB has moderate effect on P. yoelii hepatic development but is highly effective against blood stage growth. MB is active against gametocytes and abrogates parasite transmission from mice to mosquitoes. While confirming activity of MB against both sexual and asexual blood stages, the results indicate that MB has only little activity on the development of the hepatic stages of malaria parasites. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Belgium | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 21% |
| Researcher | 7 | 18% |
| Student > Doctoral Student | 3 | 8% |
| Student > Bachelor | 3 | 8% |
| Student > Master | 3 | 8% |
| Other | 3 | 8% |
| Unknown | 12 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 18% |
| Agricultural and Biological Sciences | 6 | 15% |
| Chemistry | 3 | 8% |
| Immunology and Microbiology | 2 | 5% |
| Physics and Astronomy | 2 | 5% |
| Other | 6 | 15% |
| Unknown | 13 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2023.
All research outputs
#16,159,666
of 25,540,105 outputs
Outputs from Malaria Journal
#4,157
of 5,943 outputs
Outputs of similar age
#198,517
of 343,460 outputs
Outputs of similar age from Malaria Journal
#79
of 123 outputs
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