Title |
Particulate matter 2.5 damages skin cells by inducing oxidative stress, subcellular organelle dysfunction, and apoptosis
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Published in |
Archives of Toxicology, March 2018
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DOI | 10.1007/s00204-018-2197-9 |
Pubmed ID | |
Authors |
Mei Jing Piao, Mee Jung Ahn, Kyoung Ah Kang, Yea Seong Ryu, Yu Jae Hyun, Kristina Shilnikova, Ao Xuan Zhen, Jin Woo Jeong, Yung Hyun Choi, Hee Kyoung Kang, Young Sang Koh, Jin Won Hyun |
Abstract |
The skin is the largest organ of the human body and the one mostly exposed to outdoor contaminants. To evaluate the biological mechanisms underlying skin damage caused by fine particulate matter (PM2.5), we analyzed the effects of PM2.5 on cultured human keratinocytes and the skin of experimental animals. PM2.5 was applied to human HaCaT keratinocytes at 50 µg/mL for 24 h and to mouse skin at 100 µg/mL for 7 days. The results indicate that PM2.5 induced oxidative stress by generating reactive oxygen species both in vitro and in vivo, which led to DNA damage, lipid peroxidation, and protein carbonylation. As a result, PM2.5 induced endoplasmic reticulum stress, mitochondrial swelling, and autophagy, and caused apoptosis in HaCaT cells and mouse skin tissue. The PM2.5-induced cell damage was attenuated by antioxidant N-acetyl cysteine, confirming that PM2.5 cellular toxicity was due to oxidative stress. These findings contribute to understanding of the pathophysiological mechanisms triggered in the skin by PM2.5, among which oxidative stress may play a major role. |
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Geographical breakdown
Country | Count | As % |
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Netherlands | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
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Unknown | 157 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 23 | 15% |
Researcher | 20 | 13% |
Student > Master | 18 | 11% |
Student > Bachelor | 14 | 9% |
Student > Postgraduate | 8 | 5% |
Other | 20 | 13% |
Unknown | 54 | 34% |
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Environmental Science | 12 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 8 | 5% |
Chemistry | 8 | 5% |
Other | 32 | 20% |
Unknown | 63 | 40% |