| Title |
Gene cloning and expression analysis of AhR and CYP4 from Pinctada martensii after exposed to pyrene
|
|---|---|
| Published in |
Ecotoxicology, February 2015
|
| DOI | 10.1007/s10646-015-1424-x |
| Pubmed ID | |
| Authors |
Junqiao Du, Chenghong Liao, Hailong Zhou, Xiaoping Diao, Yuhu Li, Pengfei Zheng, Fuqiang Wang |
| Abstract |
Pyrene, a typical polycyclic aromatic hydrocarbon, is a common pollutant in the marine environment. Polycyclic aromatic hydrocarbons initiate cellular detoxification in an exposed organism via the activation of the aryl hydrocarbon receptor (AhR). Subsequent metabolism of these xenobiotics is mainly by the cytochrome P450 enzymes of the phase I detoxification system. Full-length complementary DNA sequences from the pearl oyster Pinctada martensii (pm) encoding AhR and cytochrome P4 were cloned. The P. martensii AhR complementary DNA sequence constitutes an open reading frame that encodes for 848 amino acids. Sequence analysis indicated PmAhR showed high similarity with its homologues of other bivalve species. The cytochrome P(CYP)4 complementary DNA sequence of P. martensii constitutes an open reading frame that encodes for 489 amino acids. Quantitative real-time analysis detected both PmAhR and PmCYP4 messenger RNA expressions in the mantle, gill, hepatapancreas and adductor muscle of P. martensii exposed to pyrene. The highest transcript-band intensities of PmAhR and PmCYP4 were observed in the gill. Temporal expression of PmAhR and PmCYP4 messenger RNAs induction was observed in gills and increased between 3 and 5 days post exposure; then returned to control level. These results suggest that messenger RNAs of PmAhR and PmCYP4 in pearl oysters might be useful parameters for monitoring marine environment pyrene pollution. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 3 | 15% |
| Student > Ph. D. Student | 3 | 15% |
| Professor > Associate Professor | 2 | 10% |
| Researcher | 2 | 10% |
| Professor | 2 | 10% |
| Other | 3 | 15% |
| Unknown | 5 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 40% |
| Environmental Science | 1 | 5% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Earth and Planetary Sciences | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 8 | 40% |
Attention Score in Context
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All research outputs
#20,259,335
of 22,789,076 outputs
Outputs from Ecotoxicology
#970
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Outputs of similar age
#301,190
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Outputs of similar age from Ecotoxicology
#26
of 45 outputs
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