| Title |
LEUKEMIA INHIBITORY FACTOR AS AN ANTI-APOPTOTIC MITOGEN FOR PLURIPOTENT MOUSE EMBRYONIC STEM CELLS IN A SERUM-FREE MEDIUM WITHOUT FEEDER CELLS
|
|---|---|
| Published in |
In Vitro Cellular & Developmental Biology - Animal, January 2005
|
| DOI | 10.1290/0502010.1 |
| Pubmed ID | |
| Authors |
MIHO FURUE, TETSUJI OKAMOTO, YOHEI HAYASHI, HITOSHI OKOCHI, MANABU FUJIMOTO, YASUFUMI MYOISHI, TAKANORI ABE, KIYOSHI OHNUMA, GORDON H. SATO, MAKOTO ASASHIMA, J. DENRY SATO |
| Abstract |
We have developed a serum-free medium, designated ESF7, in which leukemia inhibitory factor (LIF) clearly stimulated murine embryonic stem (ES) cell proliferation accompanied by increased expression of nanog and Rex-1 and decreased FGF-5 expression. These effects were dependent on the concentration of LIF. The ES cells maintained in ESF7 medium for more than 2 yr retained an undifferentiated phenotype, as manifested by the expression of the transcription factor Oct-3/4, the stem cell marker SSEA-1, and alkaline phosphatase. Withdrawal of LIF from ESF7 medium resulted in ES cell apoptosis. Addition of serum to ESF7 medium promoted ES cell differentiation. Addition of BMP4 promoted ES cell differentiation into simple epithelial-like cells. In contrast, FGF-2 promoted ES cell differentiation into neuronal and glial-like cells. Under serum-free culture conditions, LIF was sufficient to stimulate cell proliferation, it inhibited cell differentiation, and it maintained self-renewal of ES cells. Because this simple serum-free adherent monoculture system supports the long-term propagation of pluripotent ES cells in vitro, it will allow the elucidation of ES cell responses to growth factors under defined conditions. |
Mendeley readers
The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 2% |
| France | 1 | 2% |
| Unknown | 54 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 13 | 23% |
| Student > Ph. D. Student | 11 | 20% |
| Professor > Associate Professor | 9 | 16% |
| Student > Master | 7 | 13% |
| Other | 3 | 5% |
| Other | 8 | 14% |
| Unknown | 5 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 43% |
| Biochemistry, Genetics and Molecular Biology | 16 | 29% |
| Medicine and Dentistry | 6 | 11% |
| Neuroscience | 1 | 2% |
| Chemistry | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 8 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2016.
All research outputs
#8,534,976
of 25,374,647 outputs
Outputs from In Vitro Cellular & Developmental Biology - Animal
#176
of 853 outputs
Outputs of similar age
#38,859
of 151,221 outputs
Outputs of similar age from In Vitro Cellular & Developmental Biology - Animal
#3
of 12 outputs
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