| Title |
The Role of Membrane Lateral Tension in Calcium-Induced Membrane Fusion
|
|---|---|
| Published in |
The Journal of Membrane Biology, July 2000
|
| DOI | 10.1007/s00232001076 |
| Pubmed ID | |
| Authors |
A. Chanturiya, P. Scaria, M.C. Woodle |
| Abstract |
Calcium-induced fusion of liposomes was studied with a view to understand the role of membrane tension in this process. Lipid mixing due to fusion was monitored by following fluorescence of rhodamine-phosphatidyl-ethanolamine incorporated into liposomal membrane at a self-quenching concentration. The extent of lipid mixing was found to depend on the rate of calcium addition: at slow rates it was significantly lower than when calcium was injected instantly. The vesicle inner volume was then made accessible to external calcium by adding calcium ionophore A23187. No effect on fusion was observed at high rates of calcium addition while at slow rates lipid mixing was eliminated. Fusion of labeled vesicles with a planar phospholipid membrane (BLM) was studied using fluorescence microscopy. Above a threshold concentration specific for each ion, Ca2+, Mg2+, Cd2+ and La3+ induce fusion of both charged and neutral membranes. The threshold calcium concentration required for fusion was found to be dependent on the vesicle charge, but not on the BLM charge. Pretreatment of vesicles with ionophore and calcium inhibited vesicle fusion with BLM. This effect was reversible: chelation of calcium prior to the application of vesicle to BLM completely restored their ability to fuse. These results support the hypothesis that tension in the outer monolayer of lipid vesicle is a primary reason for membrane destabilization promoting membrane fusion. How this may be a common mechanism for both purely lipidic and protein-mediated membrane fusion is discussed. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 15% |
| India | 1 | 8% |
| Unknown | 10 | 77% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 38% |
| Researcher | 2 | 15% |
| Professor | 2 | 15% |
| Other | 1 | 8% |
| Student > Doctoral Student | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 54% |
| Chemical Engineering | 2 | 15% |
| Physics and Astronomy | 2 | 15% |
| Biochemistry, Genetics and Molecular Biology | 1 | 8% |
| Unknown | 1 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 August 2013.
All research outputs
#8,534,528
of 25,373,627 outputs
Outputs from The Journal of Membrane Biology
#173
of 820 outputs
Outputs of similar age
#13,225
of 39,276 outputs
Outputs of similar age from The Journal of Membrane Biology
#1
of 6 outputs
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So far Altmetric has tracked 820 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
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