RNA Scaffolds

Chapter 1 A Method to Predict the 3D Structure of an RNA Scaffold

Chapter 2 Post-crystallization Improvement of RNA Crystal Diffraction Quality

Chapter 3 Expression and Purification of RNA–Protein Complexes in Escherichia coli

Chapter 4 Production of Homogeneous Recombinant RNA Using a tRNA Scaffold and Hammerhead Ribozymes

Chapter 5 In Vivo Production of Small Recombinant RNAs Embedded in a 5S rRNA-Derived Protective Scaffold

Chapter 6 Detection of RNA–Protein Interactions Using Tethered RNA Affinity Capture

Chapter 7 A Universal Method for Labeling Native RNA in Live Bacterial Cells

Chapter 8 Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors.

Chapter 9 RNA Scaffold: Designed to Co-localize Enzymes

Chapter 10 Artificial Ligase Ribozymes Isolated by a “Design and Selection” Strategy

Chapter 11 Engineering aptazyme switches for conditional gene expression in Mammalian cells utilizing an in vivo screening approach.

Chapter 12 Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells

Chapter 13 Design and Characterization of Topological Small RNAs.

Chapter 14 Folding RNA-Protein Complex into Designed Nanostructures.

Chapter 15 Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60° to 90° or 108°.

Chapter 16 RNA-Mediated CdS-Based Nanostructures.

Chapter 17 An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA.