| Title |
Inhibitory properties of some heavy metals on carbonic anhydrase I and II isozymes activities purified from Van Lake fish (Chalcalburnus Tarichi) gill
|
|---|---|
| Published in |
Fish Physiology and Biochemistry, April 2018
|
| DOI | 10.1007/s10695-018-0499-8 |
| Pubmed ID | |
| Authors |
Müslüm Kuzu, Veysel Çomaklı, Ebru Akkemik, Mehmet Çiftci, Ömer İrfan Küfrevioğlu |
| Abstract |
In this study, CA I and II isoenzymes were purified from Van Lake fish gills by using Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography and to determine the effects of some metals on the enzyme activities. For purified CA I isoenzyme, yield, specific activity, and purification fold were obtained as 42.07%, 4948.12 EU/mg protein, and 116.61 and for CA II isoenzyme, 7%, 1798.56 EU/mg protein, and 42.38 respectively. Activity of CA was determined by measuring "CO2-hydratase activity". Purity control was checked by SDS-PAGE. In vitro inhibitory effect of Cu2+, Ag+, Cd2+, Ni2+ metal ions, and arsenic (V) oxide were also examined for both isozymes activities. Whereas Cu2+, Ag+, Cd2+, and Ni2+ ions showed inhibitory effects on both isozymes, arsenic (V) oxide showed activation effect. IC50 values were calculated by drawing activity %-[I] graphs for metal ions exhibiting inhibitory effects. IC50 values were determined as 3.39, 6.38, 13.52, and 206 μM for CA I isozyme and 6.16, 20.29, 46, and 223 μM for CA II isozyme respectively. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 2 | 22% |
| Researcher | 2 | 22% |
| Student > Ph. D. Student | 1 | 11% |
| Student > Doctoral Student | 1 | 11% |
| Unknown | 3 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 22% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Chemistry | 1 | 11% |
| Unknown | 5 | 56% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 April 2018.
All research outputs
#14,324,809
of 23,041,514 outputs
Outputs from Fish Physiology and Biochemistry
#192
of 867 outputs
Outputs of similar age
#185,269
of 329,011 outputs
Outputs of similar age from Fish Physiology and Biochemistry
#5
of 41 outputs
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So far Altmetric has tracked 867 research outputs from this source. They receive a mean Attention Score of 1.6. This one has done well, scoring higher than 77% of its peers.
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We're also able to compare this research output to 41 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.