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Antibody Engineering

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Cover of 'Antibody Engineering'

Table of Contents

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    Book Overview
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    Chapter 1 Cloning of Variable Domains from Mouse Hybridoma by PCR
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    Chapter 2 Coning Hybridoma cDNA by RACE
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    Chapter 3 Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly
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    Chapter 4 Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens
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    Chapter 5 Human Antibody Gene Libraries
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    Chapter 6 Synthetic Antibody Libraries
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    Chapter 7 Immune Libraries from Nonhuman Primates (NHP)
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    Chapter 8 Generation of Rabbit Immune Libraries
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    Chapter 9 Immunotube Selections
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    Chapter 10 Phage Display and Selection in Microtitre Plates
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    Chapter 11 Phage Display and Selections on Biotinylated Antigens
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    Chapter 12 Phage Display and Subtractive Selection on Cells
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    Chapter 13 Selection of Phage Antibody Libraries for Binding and Internalization into Mammalian Cells
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    Chapter 14 Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA
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    Chapter 15 Yeast Display and Selections
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    Chapter 16 The Generation of Transgenic Mice Expressing Human Antibody Repertoires
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    Chapter 17 Selection of Antibody Fragments by Means of the Filter-Sandwich Colony Screening Assay
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    Chapter 18 Semi-automated Magnetic Bead-Based Antibody Selection from Phage Display Libraries
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    Chapter 19 Aspects of Isotype Selection
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    Chapter 20 Generation of Heavy and Light Chains (Chimeric Antibodies)
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    Chapter 21 Humanising Antibodies by CDR Grafting
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    Chapter 22 Humanization by Resurfacing
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    Chapter 23 Human Antibodies by Guided Selection
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    Chapter 24 In Silico De-Immunization
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    Chapter 25 Affinity Maturation by Chain Shuffling and Site Directed Mutagenesis
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    Chapter 26 Affinity Maturation by Random Mutagenesis and Phage Display
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    Chapter 27 Engineering of the Fc Region for Improved PK (FcRn Interaction)
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    Chapter 28 Antibody-Dependent Enzyme Prodrug Therapy (ADEPT)
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    Chapter 29 Production of Recombinant Human IgG Antibodies in the Baculovirus Expression System
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    Chapter 30 Expression of IgA Molecules in Mammalian Cells
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    Chapter 31 Expression of Complete Antibodies in Transgenic Plants
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    Chapter 32 Expression of Full Length Monoclonal Antibodies (mAb) in Algal Chloroplast
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    Chapter 33 Expression of IgG Antibodies in Mammalian Cells
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    Chapter 34 Protein A/G Chromatography
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    Chapter 35 Epitope Analysis Using Synthetic Peptide Repertoires Prepared by SPOT Synthesis Technology
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    Chapter 36 Epitope Mapping by Printed Peptide Libraries
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    Chapter 37 Antibody Epitope Mapping Using Yeast Display
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    Chapter 38 Size Exclusion Chromatography
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    Chapter 39 Structural Characterization of Antibodies by Mass Spectrometry
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    Chapter 40 Antibody Glycans Characterization
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    Chapter 41 Affinity Measurements by Competition ELISA
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    Chapter 42 Anti-Histidine Antibodies as Tools for Reversible Capturing of His-Tagged Fusion Proteins for Subsequent Binding Analysis
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    Chapter 43 Affinity Measurements Using Quartz Crystal Microbalance (QCM)
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    Chapter 44 Affinity Measurements with Radiolabeled Antibodies
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    Chapter 45 Neutralization Tests
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    Chapter 46 Functional Characterization of Antibodies Neutralizing Soluble Factors In Vitro and In Vivo
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    Chapter 47 Competitive ELISA
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    Chapter 48 Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
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    Chapter 49 Determination of Fc-Mediated Antibody-Effector Functions by Chromium Release Assay
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    Chapter 50 Binding Studies with Flow Cytometry
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Mentioned by

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19 patents
wikipedia
2 Wikipedia pages

Citations

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6 Dimensions

Readers on

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98 Mendeley
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Title
Antibody Engineering
Published by
Springer Berlin Heidelberg, March 2010
DOI 10.1007/978-3-642-01144-3
ISBNs
978-3-64-201143-6, 978-3-64-201144-3
Editors

Kontermann, Roland, Dübel, Stefan

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 98 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 7%
Student > Ph. D. Student 5 5%
Student > Master 2 2%
Student > Bachelor 2 2%
Professor > Associate Professor 1 1%
Other 1 1%
Unknown 80 82%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 6%
Agricultural and Biological Sciences 3 3%
Chemistry 3 3%
Pharmacology, Toxicology and Pharmaceutical Science 1 1%
Arts and Humanities 1 1%
Other 4 4%
Unknown 80 82%