| Chapter title |
Identification of sugar residues involved in the binding of TGEV to porcine brush border membranes.
|
|---|---|
| Chapter number | 22 |
| Book title |
SARS- and Other Coronaviruses
|
| Published in |
Methods in molecular biology, December 2008
|
| DOI | 10.1007/978-1-59745-181-9_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-58829-867-6, 978-1-59745-181-9
|
| Authors |
Christel Schwegmann-Wessels, Georg Herrler, Schwegmann-Wessels, Christel, Herrler, Georg |
| Abstract |
Coronaviruses most often infect the respiratory or intestinal tract. Transmissible gastroenteritis virus (TGEV), a group 1 coronavirus, infects the porcine small intestine. Piglets up to the age of 3 weeks die from diarrhea caused by the viral gastroenteritis unless they are protected by antibodies. In addition to the cellular receptor, porcine aminopeptidase N, the TGEV spike protein binds to sialic acid residues. We have shown that the sialic acid binding activity mediates the binding of TGEV to a mucin-like glycoprotein present in porcine brush border membranes. This was shown by performing a virus overlay binding assay with proteins obtained from brush border membranes by lectin precipitation. Because of the reactivity with specific lectins we assume that the recognized glycoprotein has the characteristics of a mucin. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 25% |
| Student > Master | 2 | 17% |
| Student > Ph. D. Student | 2 | 17% |
| Librarian | 1 | 8% |
| Professor | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 2 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 33% |
| Nursing and Health Professions | 3 | 25% |
| Biochemistry, Genetics and Molecular Biology | 1 | 8% |
| Unknown | 4 | 33% |