| Title |
Reactive oxygen species generation is essential for cisplatininduced accelerated senescence in hepatocellular carcinoma
|
|---|---|
| Published in |
Frontiers of Medicine, April 2014
|
| DOI | 10.1007/s11684-014-0327-1 |
| Pubmed ID | |
| Authors |
Kai Qu, Ting Lin, Zhixin Wang, Sinan Liu, Hulin Chang, Xinsen Xu, Fandi Meng, Lei Zhou, Jichao Wei, Minghui Tai, Yafeng Dong, Chang Liu |
| Abstract |
Accelerated senescence is important because this process is involved in tumor suppression and has been induced by many chemotherapeutic agents. The platinum-based chemotherapeutic agent cisplatin displays a wide range of antitumor activities. However, the molecular mechanism of cisplatin-induced accelerated senescence in hepatocellular carcinoma (HCC) remains unclear. In the present study, the growth inhibitory effect of cisplatin on HepG2 and SMMC-7721 cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cellular senescence was then assessed by β-galactosidase assay. Senescence-related factors, including p53, p21, and p16, were evaluated by quantitative reverse transcription-polymerase chain reaction. Reactive oxygen species (ROS) was analyzed by flow cytometry. Our results revealed that cisplatin reduced the proliferation of HepG2 and SMMC-7721 cells in a dose- and time-dependent manner. Senescent phenotype observed in cisplatintreated hepatoma cells was dependent on p53 and p21 activation but not on p16 activation. Furthermore, cisplatininduced accelerated senescence depended on intracellular ROS generation. The ROS scavenger N-acetyl-L-cysteine also significantly suppressed the cisplatin-induced senescence of HepG2 and SMMC-7721 cells. In conclusion, our results revealed a functional link between intracellular ROS generation and cisplatin-induced accelerated senescence, and this link may be used as a potential target of HCC. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 3% |
| Unknown | 31 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 19% |
| Student > Doctoral Student | 4 | 13% |
| Researcher | 4 | 13% |
| Professor > Associate Professor | 3 | 9% |
| Student > Bachelor | 3 | 9% |
| Other | 4 | 13% |
| Unknown | 8 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 22% |
| Agricultural and Biological Sciences | 5 | 16% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 9% |
| Medicine and Dentistry | 3 | 9% |
| Nursing and Health Professions | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 11 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2015.
All research outputs
#20,259,845
of 22,789,566 outputs
Outputs from Frontiers of Medicine
#277
of 346 outputs
Outputs of similar age
#193,267
of 227,015 outputs
Outputs of similar age from Frontiers of Medicine
#5
of 6 outputs
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