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Reviews and Protocols in DT40 Research

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Cover of 'Reviews and Protocols in DT40 Research'

Table of Contents

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    Book Overview
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    Chapter 1 DT40 gene disruptions: a how-to for the design and the construction of targeting vectors
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    Chapter 2 Immunoglobulin gene conversion or hypermutation: that's the question
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    Chapter 3 Genome resources for the DT40 community
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    Chapter 4 Chromosome engineering in DT40 cells and mammalian centromere function
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    Chapter 5 Function of RECQ family helicase in genome stability
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    Chapter 6 Genetic analysis of apoptotic execution
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    Chapter 7 The DT40 system as a tool for analyzing kinetochore assembly
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    Chapter 8 Analysing the DNA damage and replication checkpoints in DT40 cells
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    Chapter 9 Using DT40 to study clathrin function
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    Chapter 10 Genetic analysis of B cell signaling
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    Chapter 11 DT40 mutants: a model to study transcriptional regulation of B cell development and function
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    Chapter 12 Transcription and RNA processing factors play complex roles in DT40 cells
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    Chapter 13 Participation of histones, histone modifying enzymes and histone chaperonesin vertebrate cell functions
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    Chapter 14 Analysis of gene expression, copy number and palindrome formation with a DT40 enriched CDNA microarray
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    Chapter 15 Calcium signaling, ion channels and more
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    Chapter 16 Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development
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    Chapter 17 The fanconi anemia pathway promotes homologous recombination repair in DT40 cell line
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    Chapter 18 Phenotypic analysis of cellular responses to DNA damage
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    Chapter 19 ATM, a paradigm for a stress-responsive signal transducer in higher vertebrate cells
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    Chapter 20 Stable non-targeted transfection of DT40
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    Chapter 21 Basic cell culture conditions
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    Chapter 22 Excision of floxed-DNA sequences by transient induction of MER-CRE-MER
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    Chapter 23 Immunoglobulin gene conversion and hypermutation assay by facs
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    Chapter 24 Target screening by PCR
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    Chapter 25 Mitotic index determination by flow cytometry
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    Chapter 26 Centrifugal elutriation as a means of cell cycle phase separation and synchronisation
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    Chapter 27 Preparation of genomic DNA for microarray-based comparative genome hybridization
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    Chapter 28 Analysis of cellular Mg 2+ in DT40 cells
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    Chapter 29 Transient transfection of DT40
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    Chapter 30 Retroviral transduction of DT40
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    Chapter 31 Colony survival assay
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    Chapter 32 Subcloning DT40 by limiting dilution
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    Chapter 33 Subnuclear immunofluorescence
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    Chapter 34 Sister chromatid exchange assay
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    Chapter 35 2D cell cycle analysis
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    Chapter 36 Purification of tap-tagged proteins by two-step pull down from DT40 cells
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    Chapter 37 Synchronization of cells
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    Chapter 38 Targeted transfection of DT40 cells
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    Chapter 39 Luciferase reporter assay
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    Chapter 40 Indirect immunofluorescence microscopy
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    Chapter 41 Quantification of receptor-mediated endocytosis
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    Chapter 42 Measurement of DNA synthesis and strand breaks using alkaline sucrose density gradient centrifugation
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    Chapter 43 Isolation of nuclear and cytoplasmic proteins from DT40 cell lines
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Title
Reviews and Protocols in DT40 Research
Published by
Springer Netherlands, October 2006
DOI 10.1007/978-1-4020-4896-8
ISBNs
978-1-4020-4895-1, 978-1-4020-4896-8
Editors

Buerstedde, Jean-Marie, Takeda, Shunichi

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 1 3%
Unknown 30 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 35%
Student > Bachelor 8 26%
Other 2 6%
Student > Master 2 6%
Lecturer > Senior Lecturer 2 6%
Other 5 16%
Unknown 1 3%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 52%
Biochemistry, Genetics and Molecular Biology 8 26%
Environmental Science 1 3%
Immunology and Microbiology 1 3%
Medicine and Dentistry 1 3%
Other 2 6%
Unknown 2 6%