Signal Transduction Protocols

Chapter 1 Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling

Chapter 2 Imaging-Based Approaches to Understanding G Protein-Coupled Receptor Signalling Complexes

Chapter 3 Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis.

Chapter 4 RGS-Insensitive Gα Subunits: Probes of Gα Subtype-Selective Signaling and Physiological Functions of RGS Proteins

Chapter 5 Bioinformatic Approaches to Metabolic Pathways Analysis

Chapter 6 Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET

Chapter 7 Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signalling Complexes

Chapter 8 Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High-Density Lipoprotein Particles

Chapter 9 Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Heterodimerization

Chapter 10 Cell-Surface Protein–Protein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies: Application to G Protein-Coupled Receptor Oligomerization

Chapter 11 Analysis of GPCR/Ion Channel Interactions

Chapter 12 Multicolor BiFC Analysis of G Protein βγ Complex Formation and Localization

Chapter 13 Real-Time BRET Assays to Measure G Protein/Effector Interactions

Chapter 14 Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP

Chapter 15 Simultaneous Real-Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters

Chapter 16 Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity

Chapter 17 Genetically encoded fluorescent reporters to visualize protein kinase C activation in live cells.

Chapter 18 Visualizing receptor endocytosis and trafficking.

Chapter 19 Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM

Chapter 20 Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy.

Chapter 21 Detection and Characterization of Receptor Interactions with PDZ Domains

Chapter 22 Tandem Affinity Purification and Identification of Heterotrimeric G Protein-Associated Proteins

Chapter 23 Study of G Protein-Coupled Receptor/β-arrestin Interactions Within Endosomes Using FRAP

Chapter 24 Disrupting Protein Complexes Using Tat-Tagged Peptide Mimics

Chapter 25 Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein–Protein Interactions in Living Cells