DNA Repair Protocols

Table of Contents

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   Book Overview
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   Chapter 1 DNA Repair: Front and Center and Not Going Away!
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   Chapter 2 Identification of Genes Required for Damage Survival Using a Cell-Based RNAi Screen Against the Drosophila Genome.
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   Chapter 3 Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans Using Genome-Wide RNAi Screening
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   Chapter 4 Establishment of the DNA Repair-Defective Mutants in DT40 Cells
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   Chapter 5 Electrophoretic Mobility Shift Assays for Protein–DNA Complexes Involved in DNA Repair
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   Chapter 6 The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair
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   Chapter 7 Detection of DNA damage by comet fluorescence in situ hybridization.
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   Chapter 8 Quantitative DNA Damage and Repair Measurement with the Yeast Comet Assay
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    Chapter 9 Analysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR.
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    Chapter 10 In vitro DNA mismatch repair in human cells.
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    Chapter 11 Eukaryotic DNA Mismatch Repair In Vitro
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    Chapter 12 Immuno-Slot Blot Assay for Detection of UVR-Mediated DNA Damage
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    Chapter 13 Quantification of DNA Photoproducts in Mammalian Cell DNA Using Radioimmunoassay
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    Chapter 14 Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-Mediated PCR
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    Chapter 15 Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies.
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    Chapter 16 Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.
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    Chapter 17 DNA Repair Protocols
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    Chapter 18 Using Arabidopsis Cell Extracts to Monitor Repair of DNA Base Damage In Vitro
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    Chapter 19 In Vitro Monitoring of Base Excision Repair in Saccharomyces cerevisiae
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    Chapter 20 Mitochondrial Base Excision Repair Assays
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    Chapter 21 O -Hydroxylamine-Coupled Alkaline Gel Electrophoresis Assay for the Detection and Measurement of DNA Single-Strand Breaks
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    Chapter 22 DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis.
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    Chapter 23 Quantitative Amplification of Single-Stranded DNA
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    Chapter 24 Simple, Non-radioactive Measurement of Single-Stranded DNA at Telomeric, Sub-telomeric, and Genomic Loci in Budding Yeast
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    Chapter 25 Monitoring DNA Recombination Initiated by HO Endonuclease.
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    Chapter 26 Monitoring I-SceI-Induced Double-Strand Break Repair in DT40 Cells
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    Chapter 27 I-SceI-Based Assays to Examine Distinct Repair Outcomes of Mammalian Chromosomal Double Strand Breaks.
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    Chapter 28 Studying Repair of a Single Protein-Bound Nick In Vivo Using the Flp-Nick System
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    Chapter 29 Measurement of Diversification in the Immunoglobulin Light Chain Gene of DT40 Cells
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    Chapter 30 Live Cell Microscopy of DNA Damage Response in Saccharomyces cerevisiae
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    Chapter 31 Use of gene targeting to study recombination in Mammalian cell DNA repair mutants.
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    Chapter 32 In Vitro Rejoining of Double Strand Breaks in Genomic DNA
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    Chapter 33 An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides.
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    Chapter 34 Assays of Bypass Replication of Genotoxic Lesions in Cell-Free Extracts
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    Chapter 35 Quantitative measurement of translesion DNA synthesis in Mammalian cells.
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    Chapter 36 Detection of PCNA Modifications in Saccharomyces cerevisiae
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    Chapter 37 In Vitro PCNA Modification Assays
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    Chapter 38 Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40 Based In Vitro Assay of DNA Replication
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    Chapter 39 CHK1 Kinase Activity Assay
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    Chapter 40 Histone H2AX Phosphorylation: A Marker for DNA Damage.