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Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009

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Cover of 'Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009'

Table of Contents

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    Book Overview
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    Chapter 1 MicroRNAs as Potential Engineering Targets for Improvement of CHO Cell Production Phenotypes
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    Chapter 2 Are Clones Really Unstable?
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    Chapter 3 An Optimised Transfection Platform for the Epi -CHO Transient Expression System in Serum-free Media
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    Chapter 4 Using the “OMICS” Technologies as Complementary Tools to Study the Molecular Mechanisms Involved with the Adaptation of Myeloma Cell Line to Protein-Free Medium
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    Chapter 5 Galectin-3 Over-Expression Enhances Survival and Recombinant Protein Expression in Mammalian Cells
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    Chapter 6 Metabolomic Analysis of CHO Cultures with Different Growth Characteristics – Development of a Metabolite Extraction Protocol for Suspension Adapted Mammalian Cells
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    Chapter 7 Cell Lines in Four Weeks with the CEMAX ® System
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    Chapter 8 Analysis of Protein Expression via Alternate 3’ Untranslated Region (UTR) Signals Through the Use of Site Specific Recombination
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    Chapter 9 A Case Study in Clone Screening: A Comprehensive Approach for a Product With High Projected Market Demand
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    Chapter 10 An Improved Clone Selection Method
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    Chapter 11 Dissecting the Mechanism of Action of BHRF1 for the Protection Against Apoptosis in MAb-Producing Cell Lines
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    Chapter 12 Qualification of Bench and Pilot-Scale Bioreactors as Models of Commercial Systems
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    Chapter 13 Quantification of Polyethylenimine in Transient Gene Expression: On the Way to GMP Compliance
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    Chapter 14 Implications of Vector Fragmentation and Initial Production Levels in Production Cell Line Development Using MTX
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    Chapter 15 Characterizing the Expression Stability in Different Phenotypes of Recombinant NS0 Myeloma Cell Lines
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    Chapter 16 A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5 g/L in CHO Cells
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    Chapter 17 Proteomic Profiling of Temperature-Shifted CHO Cells to Identify Genes that Impact on Both Cell Growth and Recombinant Protein Productivity in Suspension Culture
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    Chapter 18 Stabilization of Protein Expression in Mammalian Cells Employing a Toxin/Antitoxin Based Strategy
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    Chapter 19 Novel Cell Lines For Bioprocessing: Friend or Foe?
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    Chapter 20 Transgene mRNA Levels and Stability are Key Factors to Enhance Transient Gene Expression in CHO DG44 Cells
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    Chapter 21 High Cell Density Transient Gene Expression in HEK 293 EBNA Cells
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    Chapter 22 Generation of High-Producing CHO Cell Lines by Piggybac Transposition
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    Chapter 23 Cellular Proteins in Conditioned Medium Inhibit Polyethylenimine-Mediated Transfection of CHO Cells
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    Chapter 24 A New Variant of the Affinity Matrix Method for Identification of High Producing Cells in Mammalian Cell Culture
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    Chapter 25 Novel Strategies for Improving Cell Viability and Production Yield
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    Chapter 26 Regulating in Vitro Motility of Human Mesenchymal Stem Cells with Macrophage Migration Inhibitory Factor (MIF) and a Small-Molecule MIF Antagonist
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    Chapter 27 Impact of Growth Factors on the Proliferation of Ear Mesenchymal Stem Cells on Porous Microcarriers
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    Chapter 28 Strategies for Pancreatic Differentiation of Pluripotent Stem Cells
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    Chapter 29 Stem Cell Biology: New Applications for Studying Metabolic Diseases
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    Chapter 30 Monitoring of Long-Term Cultivation and Osteogenic Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cell-Like Cultures
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    Chapter 31 cGMP-Compliant Isolation, Expansion and Quality Testing of Human Bone Marrow-Derived Mesenchymal Stem Cells
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    Chapter 32 Cancer Research, Molecular and Cell Biology: Look at the Nanotechnology, Based Nanoparticles for Diagnostics, and Therapy. Stem Cell for Treatment of Cancer Diseases
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    Chapter 33 A Comparative Study of Suspension Cultivation Systems for the Expansion of Undifferentiated Mouse Embryonic Stem Cells
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    Chapter 34 Human Endothelial Cell Lines with In Vivo Physiology
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    Chapter 35 Quality Control and Cell Line Cross-Contamination: An Old Problem that Continues To Fester
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    Chapter 36 Functional Characterisation of Human Hepatoma Cell Line HepG2 in 3D Culture
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    Chapter 37 GFP-Expressing Bladder Fibroblasts for Applications in Tissue Engineering
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    Chapter 38 3-Dimensional, Dynamic Cultivations of Human Umbilical Cord-Derived Cells
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    Chapter 39 NIH/3T3 Feeder System to Engineer Corneal Epithelial Sheets with Enhanced Positive Progenitor Populations
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    Chapter 40 NF-κB Signalling Pathway: Generation and Characterization of a Reporter Cell Line of Human Origin
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    Chapter 41 Establishing Mammalian Production Cell Lines for Structural Biology by Site-Specific Recombination
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    Chapter 42 Regulation of Transferrin Receptor and IGF-I Receptor Numbers at the Cell Surface Drives Growth and Productivity of Hybridoma Cells
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    Chapter 43 Towards Enhancing Manufacturing Process Performance Through Multivariate Data Mining
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    Chapter 44 Partial Replacement of Chemically Defined CHO Media with Plant-Derived Protein Hydrolysates
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    Chapter 45 An Insight into the Physiology of Insect Cells: The Role of Energetic Metabolism on the Cell Density Effect
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    Chapter 46 Design and Development of a High-Throughput Platform for Rapid Microbe Identification and Automatic Data Management
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    Chapter 47 On-line Monitoring of the Live Cell Concentration in Disposable Bioreactors
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    Chapter 48 The Challenge of Validating a Viable Biomass Probe for cGMP Processes
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    Chapter 49 Study of the Effect of High pH and Alkali Addition in a Cultivation of Chinese Hamster Ovary Cell
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    Chapter 50 Tuning of Dissolved Oxygen and pH PID Control Parameters in Large Scale Bioreactor by Lag Control
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    Chapter 51 Kinetic Analysis of Recombinant BHK Roller Bottle Cultures
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    Chapter 52 Manipulation of a Perfusion Process by Medium Optimization
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    Chapter 53 Feed Flow Pulsation in the Separation of CHO Cells in Hydrocyclones: Effects of Pressure Drop and Pumphead Type on Separation Efficiency and Cell Viability
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    Chapter 54 Development of a Biphasic Culture Process for Recombinant Protein Production in Human CAP Cells
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    Chapter 55 Crossed Mixture Design and Artificial Neural Networks: An Efficient Approach to Cell Culture Medium Optimization
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    Chapter 56 Improving Cell Culture Bioreactor Performance for Sensitive Cell Lines by Dynamic Membrane Aeration (DMA)
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    Chapter 57 Evaluation of Disposable Bioreactor Design Compared to Reusable Stainless Steel Reactors
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    Chapter 58 Utilising Scale Model Systems to Optimise Upstream Process Development
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    Chapter 59 Human Platelet Lysates as a Serum Substitute in Cell Culture Media
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    Chapter 60 4 g/L.day: Monoclonal Antibody Volumetric Productivity in the iCELLis TM Disposable Fixed-Bed Bioreactor
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    Chapter 61 Optimisation of Cell Growth and Recombinant Protein Production in Small-Scale Culture Vessels by Using Optical Sensors for On-line Measurement of Dissolved Oxygen
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    Chapter 62 A Protocol for Cell Detachment of Vero Cells Grown Under Fully Animal Component Free Conditions and on Cytodex 1 Microcarriers
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    Chapter 63 Simulation and Optimization of Essential Amino Acids in Dynamic Mammalian Cell Culture
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    Chapter 64 Biomass Sensors in iCELLis™ Fixed-Bed Reactors: Data on CHO and Vero Cells
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    Chapter 65 On-line Monitoring: Animal Cell Cultivation in iCELLis™ Fixed-Bed Reactor Using Dielectric Measurements
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    Chapter 66 Change of the Role for JAK/STAT3 in IL-6 Response During Adaptation of Hybridoma Cells to Serum-Free Media
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    Chapter 67 Engineering Principles and Cell Culture Performance of Orbitally Shaken Cylindrical Bioreactors
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    Chapter 68 Dynamic Ranking of Clones with Process Conditions Using a High-Throughput Micro-bioreactor Platform
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    Chapter 69 Improved Cell Culture Surface for Biotechnological Research
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    Chapter 70 Platform Validation of Dissolved Oxygen Ranges for Cell Culture Processes
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    Chapter 71 Study of Growth Factors in Spent Medium for Better Mammalian Cell Culture
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    Chapter 72 Process Improvements for Production of a Complex Glycosylated Fusion Protein which Delivers a Two-Fold Increase in Product Titre and Comparable Product Quality
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    Chapter 73 Hydro-Kinetic Modelling of Animal Cell Response to Turbulent Mixing in Bioreactors
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    Chapter 74 Near Infrared Spectroscopy as an In-Situ PAT Tool to Monitor Adherent VERO Cell Culture Processes
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    Chapter 75 VERO Cell Metabolism in Animal Component-Free Media: Influence of Glucose and Glutamine Substitution
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    Chapter 76 Metabolic Modeling of Drosophila melanogaster Cells Under the Balanced Growth Condition
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    Chapter 77 Mass Transfer Considerations for Scale-Up and Scale-Down of Animal Cell Bioprocesses
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    Chapter 78 Flow Characterization in Wave Bioreactors Using Computational Fluid Dynamics
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    Chapter 79 Effects of Soy Peptone-Supplemented Medium on CHO-320 Cells
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    Chapter 80 Very High Protein Production Levels with a New Transient and Serum-Free Expression System Based on Human CAP Cells
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    Chapter 81 Recombinant Albumin as an Animal-Free Supplement to Enhance Cell Culture Performance
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    Chapter 82 The Simcell TM High-Throughput Cell Culture System: An Automated Approach to Integrated Cell Line Selection and Process Development
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    Chapter 83 Advanced In Situ Microscopy for On-Line Monitoring of Animal Cell Culture
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    Chapter 84 Recombinant Antibody Yield Over 2 g/L by Transient Transfection of HEK 293 EBNA Cells in a Fed-Batch Process
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    Chapter 85 Rapid Fed-Batch Process Development in SimCell™
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    Chapter 86 Automation of Cell Line Development Using the OptiCHO Expression System
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    Chapter 87 Modeling of the Evolution of Cellular Density and Nutrient Concentrations in a Fixed-Bed Bioreactor for Its Optimization and Its Scaling-Up
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    Chapter 88 Multi-parameter Process Optimization Using the SimCell™ System
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    Chapter 89 Online Measurement of pH and O 2 Values During Mechanical Stimulation of Cells
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    Chapter 90 Reducing Protein Immunogenicity by Design: Deimmunization and Tolerance Induction
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    Chapter 91 Second-Generation Interferons: An Effective, Long-Lasting Hyperglycosylated IFN-alpha2
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    Chapter 92 Large Scale Ex Vivo Manufacture of Blood Cells
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    Chapter 93 Production of Recombinant Human Leukemia Inhibitory Factor (lif) in a Mammalian Cell Bioreactor: A First Approach
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    Chapter 94 Effect of Non-Ionic Surfactant Pluronic F-68 on CHO Cell Kinetics, IFN-γ Production and Glycosylation
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    Chapter 95 Production and Purification of a Human Dll1(ECD)IgGFc Fusion Protein in CHO SFS Cells
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    Chapter 96 Controlling Fucosylation Levels of Antibodies with Osmolality During Cell Culture in Several Host Cell Lines
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    Chapter 97 A High Cell Density Approach to Fed-Batch Cell Culture for Production of Biopharmaceuticals
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    Chapter 98 Approaches for Humanization of an Anti-idiotypic Murine Monoclonal Antibody
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    Chapter 99 Optimized CGE-LIF-Based Glycan Analysis for High-Throughput Applications
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    Chapter 100 Protein Quality Issues for Biopharmaceuticals During Solvent/Detergent Viral Inactivation Steps
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    Chapter 101 Characterisation of Cultivation and Initial Proteome Analysis of the Novel Human Cell Line AGE1.hn
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    Chapter 102 Avian Designer Cells AGE1.CR ® as Candidates for MVA and Influenza Vaccine Production
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    Chapter 103 Constitutive Augmentation of Heat Shock Response in a Producer Cell for Viral Vaccines
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    Chapter 104 In Vitro Approaches for Improved Rotavirus VLP’s Quality
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    Chapter 105 Development of Inactivated Polio Vaccine (IPV) Derived from Attenuated Sabin Strains
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    Chapter 106 Development of a Manufacturing Process for the Production of a Canine Adenovirus Type 2 (CAV-2) Vector Using MDCK Cells
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    Chapter 107 Monitoring of Host-Cell Infection and Virus-Induced Apoptosis in Influenza Vaccine Production
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    Chapter 108 Accounting for the Entire Influenza Hemagglutinin during a Flu Vaccine Production Process
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    Chapter 109 Lentiviral Vectors for Rapid and Efficient Recombinant CHO Cell Line Generation
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    Chapter 110 Thermal and Detergent Tolerance for a Chimeric Bionanoparticle
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    Chapter 111 A New Approach for Rapid Development of Spodoptera frugiperda /BEVS-Based Processes
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    Chapter 112 The Role of Culture Medium Lipids and Lipid Metabolism in Retroviral Vector Production Under Serum Deprivation
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    Chapter 113 Scale-Down of the Salk Inactivated Polio Vaccine Production Process
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    Chapter 114 Poxvirus Production on Chicken Embryo Fibroblasts in iCELLis™ Disposable Fixed-Bed Bioreactor
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    Chapter 115 Platform Technology for Viral Vaccine Production: Comparison Between Attached and Suspension Vero Cells
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    Chapter 116 Workshop 1: New Directions in Pharmaceutical Process Development and Manufacturing: Process Analytical Technology (PAT), Quality by Design (QbD), Design Space (DS), and other FDA Initiatives
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    Chapter 117 ESACT Workshop: Stem Cell Technology and Tissue Engineering New Developments and Clinical Applications
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    Chapter 118 The Impact of High-Titer Feedstreams on Monoclonal Antibody Purification
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    Chapter 119 Workshop on Genomics Technology
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Citations

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Title
Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009
Published by
Springer Netherlands, September 2011
DOI 10.1007/978-94-007-0884-6
ISBNs
978-9-40-070883-9, 978-9-40-070884-6
Editors

Jenkins, Nigel, Barron, Niall, Alves, Paula

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 100 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 1%
Switzerland 1 1%
Unknown 98 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 29 29%
Student > Master 20 20%
Student > Bachelor 14 14%
Researcher 12 12%
Student > Doctoral Student 6 6%
Other 7 7%
Unknown 12 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 34 34%
Engineering 23 23%
Biochemistry, Genetics and Molecular Biology 15 15%
Chemical Engineering 6 6%
Chemistry 4 4%
Other 4 4%
Unknown 14 14%