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Two-Hybrid Systems

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Cover of 'Two-Hybrid Systems'

Table of Contents

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    Book Overview
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    Chapter 1 Protein Interactomics by Two-Hybrid Methods
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    Chapter 2 Making the Right Choice: Critical Parameters of the Y2H Systems
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    Chapter 3 Identification of Protein–Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening
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    Chapter 4 Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System
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    Chapter 5 The Ras Recruitment System (RRS) for the Identification and Characterization of Protein–Protein Interactions
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    Chapter 6 A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway ® Cloning
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    Chapter 7 Analyses of Protein Interaction Networks Using Computational Tools
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    Chapter 8 Options and Considerations When Using a Yeast One-Hybrid System
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    Chapter 9 Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins
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    Chapter 10 Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein–DNA Interactions by High-Throughput Yeast One-Hybrid Screens
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    Chapter 11 Transcription Factor-Centered Yeast One-Hybrid Assay
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    Chapter 12 The Yeast Three-Hybrid System for Protein Interactions
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    Chapter 13 The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants
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    Chapter 14 Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules
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    Chapter 15 Shuttling of Entire Libraries from an Entry Vector to a Destination Vector of the Gateway System
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    Chapter 16 Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein–Protein Interactions in Plant Cells
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    Chapter 17 Bimolecular Fluorescence Complementation to Visualize Protein–Protein Interactions in Human Cells Based on Gateway Cloning Technology
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    Chapter 18 KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein–Protein Interactions
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    Chapter 19 Coimmunoprecipitation of Interacting Proteins in Plants
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    Chapter 20 Analysis of Protein–Protein Interaction by Co-IP in Human Cells
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    Chapter 21 Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures
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    Chapter 22 Transient Transactivation Studies in Nicotiana benthamiana Leaves
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    Chapter 23 The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein–DNA Interactions
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    Chapter 24 Chromatin Immunoprecipitation for Identification of Protein–DNA Interactions in Human Cells
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Title
Two-Hybrid Systems
Published by
Springer New York, June 2018
DOI 10.1007/978-1-4939-7871-7
ISBNs
978-1-4939-7871-7, 978-1-4939-7870-0
Editors

Oñate-Sánchez, Luis

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X Demographics

The data shown below were collected from the profiles of 24 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 26%
Student > Master 5 19%
Student > Postgraduate 3 11%
Researcher 2 7%
Student > Bachelor 1 4%
Other 3 11%
Unknown 6 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 37%
Agricultural and Biological Sciences 8 30%
Chemical Engineering 1 4%
Mathematics 1 4%
Physics and Astronomy 1 4%
Other 0 0%
Unknown 6 22%