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Plant Molecular Biology Manual

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Cover of 'Plant Molecular Biology Manual'

Table of Contents

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    Book Overview
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    Chapter 1 PEG-mediated direct gene transfer and electroporation
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    Chapter 2 Gene transfer to plants via particle bombardment
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    Chapter 3 Agrobacterium -mediated gene transfer to plant cells: cointegrate and binary vector systems
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    Chapter 4 Specialized vectors for gene tagging and expression studies
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    Chapter 5 Agrobacterium molecular genetics
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    Chapter 6 Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species
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    Chapter 7 Transient expression assays using GUS constructs and fluorometric detection for analysis of T-DNA transfer
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    Chapter 8 Agrobacterium inoculation techniques for plant tissues
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    Chapter 9 Antibiotic resistance markers for plant transformation
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    Chapter 10 Reporter genes for plants
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    Chapter 11 Opines as screenable markers for plant transformation
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    Chapter 12 Extraction of total cellular DNA from plants, algae and fungi
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    Chapter 13 Isolation and characterization of nuclear scaffolds
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    Chapter 14 Isolation of plant mitochondria and mitochondrial nucleic acids
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    Chapter 15 Isolation of chloroplasts and chloroplast DNA
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    Chapter 16 Isolation of total, poly(A) and polysomal RNA from plant tissues
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    Chapter 17 Assay for gene expression using run-on transcription in isolated nuclei
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    Chapter 18 Preparation of an in vitro transcription system of plant origin, with methods and templates for assessing its fidelity
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    Chapter 19 Southern, Northern and Western blot analysis
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    Chapter 20 Screening of cDNA expression libraries with synthetic oligonucleotides for DNA-binding proteins
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    Chapter 21 Non-radioactive nucleic acid detection systems
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    Chapter 22 RNA in situ hybridization in plants
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    Chapter 23 In situ hybridization to plant metaphase chromosomes using digoxigenin labeled nucleic acid sequences
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    Chapter 24 Methods for generating plant genomic libraries
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    Chapter 25 Construction of plant yeast artificial chromosome libraries
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    Chapter 26 Preparation of high molecular weight plant DNA and analysis by pulsed field gel electrophoresis
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    Chapter 27 Random amplified polymorphic DNA (RAPD) markers
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    Chapter 28 Gel mobility shift assay
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    Chapter 29 Optimization of DNase I footprinting experiments
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    Chapter 30 Analyses of plant chromatin and in vivo protein-DNA interactions
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    Chapter 31 Expression and characterization of recombinant plant trans-acting factors
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    Chapter 32 In vitro import of proteins into chloroplasts
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    Chapter 33 in vitro targeting of proteins to mitochondria
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    Chapter 34 Targeting of proteins to the vacuole
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    Chapter 35 Visualizing protein import into the plant cell nucleus
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    Chapter 36 Gene tagging by endogenous transposons
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    Chapter 37 Heterologous transposon tagging as a tool for the isolation of plant genes
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Title
Plant Molecular Biology Manual
Published by
Springer Netherlands, December 2012
DOI 10.1007/978-94-011-0511-8
ISBNs
978-9-40-117654-5, 978-9-40-110511-8
Editors

Gelvin, Stanton B., Schilperoort, Robbert A.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 3 23%
Student > Bachelor 3 23%
Student > Ph. D. Student 2 15%
Researcher 2 15%
Student > Doctoral Student 1 8%
Other 0 0%
Unknown 2 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 54%
Environmental Science 1 8%
Unspecified 1 8%
Immunology and Microbiology 1 8%
Chemistry 1 8%
Other 0 0%
Unknown 2 15%