| Title |
Up- and down-regulation of the mechano-gated K2P channel TREK-1 by PIP2 and other membrane phospholipids
|
|---|---|
| Published in |
Pflügers Archiv - European Journal of Physiology, March 2007
|
| DOI | 10.1007/s00424-007-0250-2 |
| Pubmed ID | |
| Authors |
Jean Chemin, Amanda Jane Patel, Fabrice Duprat, Frederick Sachs, Michel Lazdunski, Eric Honore |
| Abstract |
TREK-1 is an unconventional K(+) channel that is activated by both physical and chemical stimuli. In this study, we show that the inner leaflet membrane phospholipids, including PIP(2), exert a mixed stimulatory and inhibitory effect on TREK-1. Intra-cellular phospholipids inhibit basal channel activity and activation by membrane stretch, intra-cellular acidosis and arachidonic acid. However, binding of endogenous negative inner leaflet phospholipids with poly-lysine reduces inhibition and reveals channel stimulation by exogenous intra-cellular phospholipids. A similar effect is observed with PI, PE, PS and PA, unlike DG, demonstrating that the phosphate at position 3 is required although the global charge of the molecule is not critical. Inhibition depends on the distal C-terminal domain that conditions channel mechano-sensitivity, but is independent of the positively charged PIP(2) stimulatory site in the proximal C-terminal domain. This is, to our knowledge, the first report of an ion channel dually regulated by membrane phospholipids. |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 3% |
| United Kingdom | 2 | 3% |
| Japan | 1 | 2% |
| Unknown | 56 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 28% |
| Researcher | 13 | 21% |
| Student > Bachelor | 6 | 10% |
| Student > Master | 5 | 8% |
| Student > Postgraduate | 4 | 7% |
| Other | 11 | 18% |
| Unknown | 5 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 38% |
| Biochemistry, Genetics and Molecular Biology | 15 | 25% |
| Medicine and Dentistry | 5 | 8% |
| Engineering | 3 | 5% |
| Neuroscience | 3 | 5% |
| Other | 8 | 13% |
| Unknown | 4 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2013.
All research outputs
#7,855,444
of 23,815,455 outputs
Outputs from Pflügers Archiv - European Journal of Physiology
#476
of 1,973 outputs
Outputs of similar age
#28,061
of 78,340 outputs
Outputs of similar age from Pflügers Archiv - European Journal of Physiology
#6
of 11 outputs
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