| Title |
Establishment and proteomic characterization of a novel synovial sarcoma cell line, NCC-SS2-C1
|
|---|---|
| Published in |
In Vitro Cellular & Developmental Biology - Animal, April 2018
|
| DOI | 10.1007/s11626-018-0237-7 |
| Pubmed ID | |
| Authors |
Rieko Oyama, Fusako Kito, Marimu Sakumoto, Kumiko Shiozawa, Shunichi Toki, Makoto Endo, Akihiko Yoshida, Akira Kawai, Tadashi Kondo |
| Abstract |
Synovial sarcoma is an aggressive mesenchymal tumor, characterized by the presence of unique transfusion gene, SS18-SSX. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of SS18-SSX in relevant cellular contexts. We report the establishment and proteomic characterization of a novel synovial sarcoma cell line. Primary tissue culture was performed using tumor tissue of synovial sarcoma. The established cell line was authenticated by assessing its DNA microsatellite short tandem repeat analysis and characterized by in vitro assay. Proteomic study was achieved by mass spectrometry, and the results were analyzed by treemap. The cell line NCC-SS2-C1 was established from a primary tumor tissue of a synovial sarcoma patient. The cell line has grown well for 11 mo and has been subcultured more than 15 times. The established cells were authenticated by assessing their short tandem repeat pattern comparing with that of original tumor tissue. The cells showed polygonal in shape and formed spheroid when seeded on the low-attachment dish. Proteomic analysis revealed the molecular pathways which are unique to the original tumor tissue or the established cell line. In conclusion, a novel synovial sarcoma cell line NCC-SS2-C1 was successfully established from the primary tumor tissue. The cell line has characteristic transfusion SS18-SSX and poses aggressive in vitro growth and capability of spheroid formation. Thus, NCC-SS2-C1 cell line will be a useful tool for investigation of the mechanisms of disease and the biological role of fusion gene. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 25% |
| Italy | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 25% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 4 | 57% |
| Student > Ph. D. Student | 1 | 14% |
| Other | 1 | 14% |
| Student > Postgraduate | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 4 | 57% |
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Immunology and Microbiology | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2018.
All research outputs
#14,387,654
of 23,041,514 outputs
Outputs from In Vitro Cellular & Developmental Biology - Animal
#452
of 797 outputs
Outputs of similar age
#187,085
of 329,529 outputs
Outputs of similar age from In Vitro Cellular & Developmental Biology - Animal
#3
of 10 outputs
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