| Title |
Human sterol 12α-hydroxylase (CYP8B1) is mainly expressed in hepatocytes in a homogenous pattern
|
|---|---|
| Published in |
Histochemistry and Cell Biology, May 2005
|
| DOI | 10.1007/s00418-005-0779-0 |
| Pubmed ID | |
| Authors |
Jin Wang, Sinead Greene, Lennart C. Eriksson, Björn Rozell, Eva Reihnér, Curt Einarsson, Gösta Eggertsen, Mats Gåfvels |
| Abstract |
The liver is the only organ where the complete synthesis of bile acids takes place. The present study was undertaken to investigate whether regional differences exist within the individual human hepatic lobuli regarding the pattern of expression of sterol 12alpha-hydroxylase (CYP8B1), a key enzyme in bile acid synthesis. A specific anti-human CYP8B1 peptide antiserum was developed and used for Western blotting and hepatic immunostaining of livers from various patients. CYP8B1 in human liver was expressed in the cytoplasm of hepatocytes with an even nonzonal distribution within the liver lobulus. Pericentral expression was confirmed for CYP2E1. A weak staining was noted in cholangiocytes and Kupffer cells. Previous studies on hepatic CYP27A1 and CYP7A1 in rats have shown a zonal expression, primarily in the pericentral region. Our studies indicate a different pattern for CYP8B1 expression in human liver, which was even rather than zonal. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 14% |
| Unknown | 6 | 86% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 43% |
| Researcher | 2 | 29% |
| Student > Doctoral Student | 1 | 14% |
| Student > Master | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 43% |
| Agricultural and Biological Sciences | 2 | 29% |
| Chemistry | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
All research outputs
#8,064,660
of 24,217,893 outputs
Outputs from Histochemistry and Cell Biology
#247
of 926 outputs
Outputs of similar age
#21,372
of 60,158 outputs
Outputs of similar age from Histochemistry and Cell Biology
#3
of 5 outputs
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So far Altmetric has tracked 926 research outputs from this source. They receive a mean Attention Score of 3.6. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
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