RT-PCR Protocols

Chapter 1 Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology

Chapter 2 Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer

Chapter 3 Transcriptome Profiling of Host–Microbe Interactions by Differential Display RT-PCR

Chapter 4 Quantitative RT-PCR Methods for Mature microRNA Expression Analysis

Chapter 5 Detection of Influenza A Virus Neuraminidase and PB2 Gene Segments by One Step Reverse Transcription Polymerase Chain Reaction

Chapter 6 Detection and Identification of CD46 Splicing Isoforms by Nested RT-PCR

Chapter 7 Simultaneous Detection of Bluetongue Virus RNA, Internal Control GAPDH mRNA, and External Control Synthetic RNA by Multiplex Real-Time PCR

Chapter 8 RT-PCR Protocols

Chapter 9 Detection of antisense RNA transcripts by strand-specific RT-PCR.

Chapter 10 RT-PCR Amplification and Cloning of Large Viral Sequences

Chapter 11 One-Step RT-LATE-PCR for mRNA and Viral RNA Detection and Quantification

Chapter 12 Changes in Gene Expression of Caveolin-1 After Inflammatory Pain Using Quantitative Real-Time PCR

Chapter 13 Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction

Chapter 14 The use of comparative quantitative RT-PCR to investigate the effect of cysteine incubation on GPx1 expression in freshly isolated cardiomyocytes.

Chapter 15 The Renaissance of Competitive PCR as an Accurate Tool for Precise Nucleic Acid Quantification

Chapter 16 Skeletal Muscle RNA Extraction in Preparation for RT-PCR

Chapter 17 Reverse Transcription of the Ribonucleic Acid: The First Step in RT-PCR Assay

Chapter 18 Primer Design for RT-PCR

Chapter 19 Hot Start PCR

Chapter 20 Real-time RT-PCR for Automated Detection of HIV-1 RNA During Blood Donor Screening