| Title |
Novel compounds that enhance Agrobacterium-mediated plant transformation by mitigating oxidative stress
|
|---|---|
| Published in |
Plant Cell Reports, November 2014
|
| DOI | 10.1007/s00299-014-1707-3 |
| Pubmed ID | |
| Authors |
Yinghui Dan, Song Zhang, Heng Zhong, Hochul Yi, Manuel B. Sainz |
| Abstract |
Agrobacterium tumefaciens caused tissue browning leading to subsequent cell death in plant transformation and novel anti-oxidative compounds enhanced Agrobacterium -mediated plant transformation by mitigating oxidative stress. Browning and death of cells transformed with Agrobacterium tumefaciens is a long-standing and high impact problem in plant transformation and the agricultural biotechnology industry, severely limiting the production of transgenic plants. Using our tomato cv. MicroTom transformation system, we demonstrated that Agrobacterium caused tissue browning (TB) leading to subsequent cell death by our correlation study. Without an antioxidant (lipoic acid, LA) TB was severe and associated with high levels of GUS transient expression and low stable transformation frequency (STF). LA addition shifted the curve in that most TB was intermediate and associated with the highest levels of GUS transient expression and STF. We evaluated 18 novel anti-oxidative compounds for their potential to enhance Agrobacterium-mediated transformation, by screening for TB reduction and monitoring GUS transient expression. Promising compounds were further evaluated for their effect on MicroTom and soybean STF. Among twelve non-antioxidant compounds, seven and five significantly (P < 0.05) reduced TB and increased STF, respectively. Among six antioxidants four of them significantly reduced TB and five of them significantly increased STF. The most efficient compound found to increase STF was melatonin (MEL, an antioxidant). Optimal concentrations and stages to use MEL in transformation were determined, and Southern blot analysis showed that T-DNA integration was not affected by MEL. The ability of diverse compounds with different anti-oxidative mechanisms can reduce Agrobacterium-mediated TB and increase STF, strongly supporting that oxidative stress is an important limiting factor in Agrobacterium-mediated transformation and the limiting factor can be controlled by these compounds at different levels. |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 2% |
| Spain | 1 | 2% |
| Italy | 1 | 2% |
| Unknown | 54 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 30% |
| Student > Master | 10 | 18% |
| Student > Ph. D. Student | 5 | 9% |
| Student > Bachelor | 4 | 7% |
| Lecturer | 3 | 5% |
| Other | 4 | 7% |
| Unknown | 14 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 25 | 44% |
| Biochemistry, Genetics and Molecular Biology | 12 | 21% |
| Nursing and Health Professions | 2 | 4% |
| Immunology and Microbiology | 1 | 2% |
| Medicine and Dentistry | 1 | 2% |
| Other | 2 | 4% |
| Unknown | 14 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 February 2015.
All research outputs
#15,322,526
of 22,789,566 outputs
Outputs from Plant Cell Reports
#1,739
of 2,182 outputs
Outputs of similar age
#214,350
of 361,938 outputs
Outputs of similar age from Plant Cell Reports
#10
of 27 outputs
Altmetric has tracked 22,789,566 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,182 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 361,938 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.