| Title |
Fluorescent Function-Spacer-Lipid Construct Labelling Allows for Real-Time in Vivo Imaging of Cell Migration and Behaviour in Zebrafish (Danio Rerio)
|
|---|---|
| Published in |
Journal of Fluorescence, March 2012
|
| DOI | 10.1007/s10895-012-1043-3 |
| Pubmed ID | |
| Authors |
Chuan-Ching Lan, Deborah Blake, Stephen Henry, Donald R. Love |
| Abstract |
Real-time in vivo imaging of cell migration and behavior has advanced our understanding of physiological processes in situ, especially in the field of immunology. We carried out the transplantation of a mixed population of blood cells from adult zebrafish (Danio rerio) to 2 day old embryos. The blood cells were treated ex vivo with Function-Spacer-Lipid constructs (FSL) incorporating either fluorescein or Atto488 fluorophores (FSL-FLRO4-I or -II). Excellent labeling efficiency was demonstrated by epifluorescence microscopy and FACScan analysis. Real-time video imaging of the recipient fish showed that the functionality of these cells was retained and not affected by the labeling. The usefulness of FSL-FLRO4-I as a contrast agent in microangiography was explored. Overall, we found both FSL-FLRO4-I and-II promising labeling dyes for real-time in vivo imaging in zebrafish. |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 7% |
| Unknown | 14 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 27% |
| Student > Ph. D. Student | 4 | 27% |
| Lecturer | 1 | 7% |
| Student > Doctoral Student | 1 | 7% |
| Librarian | 1 | 7% |
| Other | 3 | 20% |
| Unknown | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 40% |
| Medicine and Dentistry | 3 | 20% |
| Biochemistry, Genetics and Molecular Biology | 2 | 13% |
| Environmental Science | 1 | 7% |
| Physics and Astronomy | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 1 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2012.
All research outputs
#7,454,427
of 22,789,566 outputs
Outputs from Journal of Fluorescence
#98
of 425 outputs
Outputs of similar age
#52,688
of 160,855 outputs
Outputs of similar age from Journal of Fluorescence
#2
of 3 outputs
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So far Altmetric has tracked 425 research outputs from this source. They receive a mean Attention Score of 3.0. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
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