MAP Kinase Signaling Protocols

Chapter 1 The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of Physiological Functions

Chapter 2 Determination of ERK Activity: Anti-phospho-ERK Antibodies and In Vitro Phosphorylation

Chapter 3 Activation of SAPK/JNKs In Vitro

Chapter 4 Activation of p38 and Determination of Its Activity

Chapter 5 Activity Assays for Extracellular Signal-Regulated Kinase 5

Chapter 6 Use of inhibitors in the study of MAP kinases.

Chapter 7 MAP Kinase activation by receptor tyrosine kinases: in control of cell migration.

Chapter 8 Activation of Ras and Rho GTPases and MAP Kinases by G-Protein-Coupled Receptors

Chapter 9 Regulation of MAP Kinase Signaling by Calcium

Chapter 10 Identification of Novel Substrates of MAP Kinase Cascades Using Bioengineered Kinases that Uniquely Utilize Analogs of ATP to Phosphorylate Substrates

Chapter 11 ERK-MAP Kinase Signaling in the Cytoplasm

Chapter 12 Lentiviral Vectors to Study the Differential Function of ERK1 and ERK2 MAP Kinases

Chapter 13 Structural Studies of MAP Kinase Cascade Components

Chapter 14 MAP Kinase Signaling Protocols

Chapter 15 A “Molecular Evolution” Approach for Isolation of Intrinsically Active (MEK-Independent) MAP Kinases

Chapter 16 Reconstitution of the Nuclear Transport of the MAP Kinase ERK2

Chapter 17 Localization and Trafficking of Fluorescently Tagged ERK1 and ERK2

Chapter 18 Studying the Regulation of MAP Kinase by MAP Kinase Phosphatases In Vitro and in Cell Systems

Chapter 19 Proteomic analysis of scaffold proteins in the ERK cascade.

Chapter 20 Analysis of ERKs’ Dimerization by Electrophoresis

Chapter 21 MAP Kinase: SUMO pathway interactions.

Chapter 22 Computational modelling of kinase signalling cascades.

Chapter 23 Analysis of Mitogen-Activated Protein Kinase Activity in Yeast

Chapter 24 Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining

Chapter 25 Studying MAP Kinase Pathways During Early Development of Xenopus laevis

Chapter 26 Deciphering Signaling Pathways In Vivo: The Ras/Raf/Mek/Erk Cascade

Chapter 27 Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes

Chapter 28 Implication of the ERK Pathway on the Post-transcriptional Regulation of VEGF mRNA Stability

Chapter 29 Studies on MAP Kinase signaling in the immune system.

Chapter 30 Methods to Study MAP Kinase Signalling in the Central Nervous System

Chapter 31 MAP Kinase Regulation of the Mitotic Spindle Checkpoint

Chapter 32 Using High-Content Microscopy to Study Gonadotrophin-Releasing Hormone Regulation of ERK