| Title |
High-Level Expression of the Antimicrobial Peptide Plectasin in Escherichia coli
|
|---|---|
| Published in |
Current Microbiology, February 2010
|
| DOI | 10.1007/s00284-010-9596-3 |
| Pubmed ID | |
| Authors |
Xiao-Lan Jing, Xue-Gang Luo, Wen-Jing Tian, Li-Hui Lv, Yong Jiang, Nan Wang, Tong-Cun Zhang |
| Abstract |
Plectasin is a defensin-like antimicrobial peptide isolated from a fungus, the saprophytic ascomycete Pseudoplectania nigrella. Plectasin showed marked antibacterial activity in vitro against Gram-positive bacteria, especially Streptococcus pneumoniae, including strains resistant to conventional antibiotics. Plectasin could kill the sensitive strain as efficaciously as vancomycin and penicillin and without cytotoxic effects on mammalian cell viability. In order to establish a bacterium-based plectasin production system, in the present study, the coding sequence of plectasin was optimized, and then cloned into pET32a (+) vector and expressed as a thioredoxin (Trx) fusion protein in Escherichia coli. The soluble fusion protein collected from the supernatant of the cell lysate was separated by Ni(2+)-chelating affinity chromatography. The purified protein was then cleaved by Factor Xa protease to release mature plectasin. Final purification was achieved by Ni(2+)-chelating chromatography again. The recombinant plectasin exhibited the same antimicrobial activity as reported previously. This is the first study to describe the expression of plectasin in E. coli expression system, and these works might provide a significant foundation for the following production or study of plectasin, and contribute to the development and evolution of novel antimicrobial drugs in clinical applications. |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 33% |
| Researcher | 6 | 20% |
| Student > Bachelor | 3 | 10% |
| Professor | 2 | 7% |
| Student > Master | 2 | 7% |
| Other | 2 | 7% |
| Unknown | 5 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 40% |
| Biochemistry, Genetics and Molecular Biology | 8 | 27% |
| Environmental Science | 1 | 3% |
| Veterinary Science and Veterinary Medicine | 1 | 3% |
| Arts and Humanities | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 6 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 November 2011.
All research outputs
#4,696,781
of 22,789,566 outputs
Outputs from Current Microbiology
#201
of 2,409 outputs
Outputs of similar age
#20,236
of 94,263 outputs
Outputs of similar age from Current Microbiology
#2
of 7 outputs
Altmetric has tracked 22,789,566 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,409 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 94,263 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.