RNA Nanotechnology and Therapeutics

Chapter 1 Overview of methods in RNA nanotechnology: synthesis, purification, and characterization of RNA nanoparticles.

Chapter 2 Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-assembly.

Chapter 3 Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM.

Chapter 4 Silver nanoclusters for RNA nanotechnology: steps towards visualization and tracking of RNA nanoparticle assemblies.

Chapter 5 Large scale purification of RNA nanoparticles by preparative ultracentrifugation.

Chapter 6 HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single-Nucleotide Resolution

Chapter 7 Using RNA nanoparticles with thermostable motifs and fluorogenic modules for real-time detection of RNA folding and turnover in prokaryotic and eukaryotic cells.

Chapter 8 Fluorescence Labeling of Short RNA by Oxidation at the 3′-End

Chapter 9 Methods and assays for specific targeting and delivery of RNA nanoparticles to cancer metastases.

Chapter 10 Functional assays for specific targeting and delivery of RNA nanoparticles to brain tumor.

Chapter 11 Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide–Protein Complexes to SELEX Screens

Chapter 12 Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge.

Chapter 13 A high-throughput screening assay for the functional delivery of splice-switching oligonucleotides in human melanoma cells.

Chapter 14 Design, Assembly, and Evaluation of RNA-Protein Nanostructures.

Chapter 15 Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq

Chapter 16 Mapping Protein–RNA Interactions by RCAP, RNA-Cross-Linking and Peptide Fingerprinting