Cystic Fibrosis

Chapter 1 Introduction to Section I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology

Chapter 2 Imaging CFTR Protein Localization in Cultured Cells and Tissues

Chapter 3 Cystic Fibrosis

Chapter 4 Methods for Evaluating Inflammation in Cystic Fibrosis

Chapter 5 Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures

Chapter 6 Measurement of Fluid Secretion from Intact Airway Submucosal Glands

Chapter 7 Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia

Chapter 8 Identification and Quantification of Mucin Expression

Chapter 9 Methods to Classify Bacterial Pathogens in Cystic Fibrosis

Chapter 10 Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells

Chapter 11 Introduction to Section II: Omics in the Biology of Cystic Fibrosis

Chapter 12 Microarray mRNA Expression Profiling to Study Cystic Fibrosis

Chapter 13 Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture)

Chapter 14 Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis

Chapter 15 Functional Genomics Assays to Study CFTR Traffic and ENaC Function

Chapter 16 New Lipidomic Approaches in Cystic Fibrosis

Chapter 17 Introduction to Section III: Resources for CFTR Research

Chapter 18 Primary Epithelial Cell Models for Cystic Fibrosis Research

Chapter 19 Comparative Biology of Cystic Fibrosis Animal Models

Chapter 20 CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction

Chapter 21 Evaluation of the Disease Liability of CFTR Variants