| Title |
Chymotrypsin gene expression in rat peripheral organs
|
|---|---|
| Published in |
Cell and Tissue Research, April 1998
|
| DOI | 10.1007/s004410051065 |
| Pubmed ID | |
| Authors |
Xiao-Cun Wang, Kenneth I. Strauss, Quy N. Ha, Satish Nagula, Matthew E. Wolpoe, David M. Jacobowitz |
| Abstract |
Prior studies have revealed the presence of chymotrypsinlike protease in peripheral organs, although no definitive evidence for the synthesis of this enzyme in tissue other than the pancreas is available. In an attempt to detect chymotrypsinogen mRNA in peripheral organs, a fragment of the pancreatic chymotrypsin mRNA from rat was amplified using PCR. The sequence was identified as a portion of the rat chymotrypsin B gene overlapping exon 5 through exon 7. It was subcloned into the pGEM-4Z vector and used as a template for the vitro transcription of an antisense riboprobe. Using ribonuclease protection and Northern blot analyses, chymotrypsin mRNA was detected in the rat pancreas, stomach, duodenum, ovary, and spleen. Monoclonal and polyclonal antisera against chymotrypsin detected chymotrypsinlike immunoreactivity in rat and human pancreas, rat stomach, duodenum and jejunum. Electrophoresis and immunoblotting revealed chymotrypsin-chymotrypsinogen bands (25-29 kDa) in the stomach and duodenum. Synthesis of a potent protease such as chymotrypsin in tissue other than pancreas is significant, suggesting a potential physiological and/or pathological role in these tissues. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 1 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 1 | 100% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 February 2010.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Cell and Tissue Research
#546
of 2,232 outputs
Outputs of similar age
#10,369
of 32,424 outputs
Outputs of similar age from Cell and Tissue Research
#3
of 12 outputs
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