| Title |
Increased Ifi202b/IFI16 expression stimulates adipogenesis in mice and humans
|
|---|---|
| Published in |
Diabetologia, February 2018
|
| DOI | 10.1007/s00125-018-4571-9 |
| Pubmed ID | |
| Authors |
Mandy Stadion, Kristin Schwerbel, Antonia Graja, Christian Baumeier, Maria Rödiger, Wenke Jonas, Christian Wolfrum, Harald Staiger, Andreas Fritsche, Hans-Ulrich Häring, Nora Klöting, Matthias Blüher, Pamela Fischer-Posovszky, Tim J. Schulz, Hans-Georg Joost, Heike Vogel, Annette Schürmann |
| Abstract |
Obesity results from a constant and complex interplay between environmental stimuli and predisposing genes. Recently, we identified the IFN-activated gene Ifi202b as the most likely gene responsible for the obesity quantitative trait locus Nob3 (New Zealand Obese [NZO] obesity 3). The aim of this study was to evaluate the effects of Ifi202b on body weight and adipose tissue biology, and to clarify the functional role of its human orthologue IFI16. The impact of Ifi202b and its human orthologue IFI16 on adipogenesis was investigated by modulating their respective expression in murine 3T3-L1 and human Simpson-Golabi-Behmel syndrome (SGBS) pre-adipocytes. Furthermore, transgenic mice overexpressing IFI202b were generated and characterised with respect to metabolic traits. In humans, expression levels of IFI16 in adipose tissue were correlated with several variables of adipocyte function. In mice, IFI202b overexpression caused obesity (Δ body weight at the age of 30 weeks: 10.2 ± 1.9 g vs wild-type mice) marked by hypertrophic fat mass expansion, increased expression of Zfp423 (encoding the transcription factor zinc finger protein [ZFP] 423) and white-selective genes (Tcf21, Tle3), and decreased expression of thermogenic genes (e.g. Cidea, Ucp1). Compared with their wild-type littermates, Ifi202b transgenic mice displayed lower body temperature, hepatosteatosis and systemic insulin resistance. Suppression of IFI202b/IFI16 in pre-adipocytes impaired adipocyte differentiation and triacylglycerol storage. Humans with high levels of IFI16 exhibited larger adipocytes, an enhanced inflammatory state and impaired insulin-stimulated glucose uptake in white adipose tissue. Our findings reveal novel functions of Ifi202b and IFI16, demonstrating their role as obesity genes. These genes promote white adipogenesis and fat storage, thereby facilitating the development of obesity-associated insulin resistance. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 40% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 80% |
| Practitioners (doctors, other healthcare professionals) | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 38% |
| Student > Doctoral Student | 4 | 13% |
| Researcher | 3 | 9% |
| Student > Bachelor | 2 | 6% |
| Professor | 2 | 6% |
| Other | 4 | 13% |
| Unknown | 5 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 28% |
| Biochemistry, Genetics and Molecular Biology | 6 | 19% |
| Sports and Recreations | 2 | 6% |
| Neuroscience | 2 | 6% |
| Medicine and Dentistry | 2 | 6% |
| Other | 3 | 9% |
| Unknown | 8 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 October 2023.
All research outputs
#6,299,448
of 24,784,213 outputs
Outputs from Diabetologia
#2,631
of 5,300 outputs
Outputs of similar age
#102,522
of 335,890 outputs
Outputs of similar age from Diabetologia
#46
of 64 outputs
Altmetric has tracked 24,784,213 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
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