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Mendeley readers
Attention Score in Context
| Title |
Alanine Scanning Mutagenesis of the Prototypic Cyclotide Reveals a Cluster of Residues Essential for Bioactivity*
|
|---|---|
| Published in |
Journal of Biological Chemistry, February 2008
|
| DOI | 10.1074/jbc.m709303200 |
| Pubmed ID | |
| Authors |
Shane M. Simonsen, Lillian Sando, K. Johan Rosengren, Conan K. Wang, Michelle L. Colgrave, Norelle L. Daly, David J. Craik |
| Abstract |
The cyclotides are stable plant-derived mini-proteins with a topologically circular peptide backbone and a knotted arrangement of three disulfide bonds that form a cyclic cystine knot structural framework. They display a wide range of pharmaceutically important bioactivities, but their natural function is in plant defense as insecticidal agents. To determine the influence of individual residues on structure and activity in the prototypic cyclotide kalata B1, all 23 non-cysteine residues were successively replaced with alanine. The structure was generally tolerant of modification, indicating that the framework is a viable candidate for the stabilization of bioactive peptide epitopes. Remarkably, insecticidal and hemolytic activities were both dependent on a common, well defined cluster of hydrophilic residues on one face of the cyclotide. Interestingly, this cluster is separate from the membrane binding face of the cyclotides. Overall, the mutagenesis data provide an important insight into cyclotide biological activity and suggest that specific self-association, in combination with membrane binding mediates cyclotide bioactivities. |
Mendeley readers
The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| New Zealand | 1 | 1% |
| Russia | 1 | 1% |
| Australia | 1 | 1% |
| Unknown | 88 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 23% |
| Researcher | 17 | 19% |
| Student > Bachelor | 10 | 11% |
| Student > Master | 7 | 8% |
| Student > Doctoral Student | 4 | 4% |
| Other | 14 | 15% |
| Unknown | 18 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 26 | 29% |
| Agricultural and Biological Sciences | 21 | 23% |
| Chemistry | 18 | 20% |
| Medicine and Dentistry | 3 | 3% |
| Mathematics | 1 | 1% |
| Other | 4 | 4% |
| Unknown | 18 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 July 2019.
All research outputs
#5,446,629
of 25,373,627 outputs
Outputs from Journal of Biological Chemistry
#13,967
of 85,238 outputs
Outputs of similar age
#25,093
of 172,217 outputs
Outputs of similar age from Journal of Biological Chemistry
#77
of 404 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 85,238 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 172,217 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 404 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.