| Title |
Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell tracking and traction force microscopy
|
|---|---|
| Published in |
Cellular and Molecular Life Sciences, December 2011
|
| DOI | 10.1007/s00018-011-0893-1 |
| Pubmed ID | |
| Authors |
Sara Nocentini, Diego Reginensi, Simón Garcia, Patricia Carulla, María Teresa Moreno-Flores, Francisco Wandosell, Xavier Trepat, Ana Bribian, José A. del Río |
| Abstract |
Newly generated olfactory receptor axons grow from the peripheral to the central nervous system aided by olfactory ensheathing cells (OECs). Thus, OEC transplantation has emerged as a promising therapy for spinal cord injuries and for other neural diseases. However, these cells do not present a uniform population, but instead a functionally heterogeneous population that exhibits a variety of responses including adhesion, repulsion, and crossover during cell-cell and cell-matrix interactions. Some studies report that the migratory properties of OECs are compromised by inhibitory molecules and potentiated by chemical gradients. Here, we demonstrated that rodent OECs express all the components of the Nogo receptor complex and that their migration is blocked by myelin. Next, we used cell tracking and traction force microscopy to analyze OEC migration and its mechanical properties over myelin. Our data relate the decrease of traction force of OEC with lower migratory capacity over myelin, which correlates with changes in the F-actin cytoskeleton and focal adhesion distribution. Lastly, OEC traction force and migratory capacity is enhanced after cell incubation with the Nogo receptor inhibitor NEP1-40. |
Mendeley readers
The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 3% |
| Spain | 1 | 2% |
| India | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 57 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 34% |
| Researcher | 10 | 16% |
| Student > Bachelor | 7 | 11% |
| Professor > Associate Professor | 4 | 6% |
| Student > Doctoral Student | 3 | 5% |
| Other | 9 | 15% |
| Unknown | 8 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 25 | 40% |
| Neuroscience | 12 | 19% |
| Biochemistry, Genetics and Molecular Biology | 6 | 10% |
| Materials Science | 4 | 6% |
| Engineering | 3 | 5% |
| Other | 5 | 8% |
| Unknown | 7 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 December 2014.
All research outputs
#7,845,540
of 23,794,258 outputs
Outputs from Cellular and Molecular Life Sciences
#1,655
of 4,151 outputs
Outputs of similar age
#72,441
of 248,441 outputs
Outputs of similar age from Cellular and Molecular Life Sciences
#6
of 19 outputs
Altmetric has tracked 23,794,258 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,151 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 248,441 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.