| Title |
3-Hydroxyglutaric acid is transported via the sodium-dependent dicarboxylate transporter NaDC3
|
|---|---|
| Published in |
Journal of Molecular Medicine, March 2007
|
| DOI | 10.1007/s00109-007-0174-5 |
| Pubmed ID | |
| Authors |
Franziska Stellmer, Britta Keyser, Birgitta C. Burckhardt, Hermann Koepsell, Thomas Streichert, Markus Glatzel, Sabrina Jabs, Joachim Thiem, Wilhelm Herdering, David M. Koeller, Stephen I. Goodman, Zoltan Lukacs, Kurt Ullrich, Gerhard Burckhardt, Thomas Braulke, Chris Mühlhausen |
| Abstract |
Patients with glutaryl-CoA dehydrogenase (GCDH) deficiency accumulate glutaric acid (GA) and 3-hydroxyglutaric acid (3OH-GA) in their blood and urine. To identify the transporter mediating the translocation of 3OH-GA through membranes, kidney tissue of Gcdh-/- mice have been investigated because of its central role in urinary excretion of this metabolite. Using microarray analyses of kidney-expressed genes in Gcdh-/- mice, several differentially expressed genes encoding transporter proteins were identified. Real-time polymerase chain reaction analysis confirmed the upregulation of the sodium-dependent dicarboxylate cotransporter 3 (NaDC3) and the organic cation transporter 2 (OCT2). Expression analysis of NaDC3 in Xenopus laevis oocytes by the two-electrode-voltage-clamp technique demonstrated the sodium-dependent translocation of 3OH-GA with a K (M) value of 0.95 mM. Furthermore, tracer flux measurements in Chinese hamster ovary cells overexpressing OCT2 showed that 3OH-GA inhibited significantly the uptake of methyl-4-phenylpyridinium, whereas 3OH-GA is not transported by OCT2. The data demonstrate for the first time the membrane translocation of 3OH-GA mediated by NaDC3 and the cis-inhibitory effect on OCT2-mediated transport of cations. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 15% |
| Unspecified | 2 | 8% |
| Researcher | 2 | 8% |
| Professor > Associate Professor | 2 | 8% |
| Student > Doctoral Student | 2 | 8% |
| Other | 9 | 35% |
| Unknown | 5 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 23% |
| Agricultural and Biological Sciences | 5 | 19% |
| Medicine and Dentistry | 4 | 15% |
| Unspecified | 2 | 8% |
| Immunology and Microbiology | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 5 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2017.
All research outputs
#4,696,781
of 22,790,780 outputs
Outputs from Journal of Molecular Medicine
#230
of 1,551 outputs
Outputs of similar age
#14,499
of 75,884 outputs
Outputs of similar age from Journal of Molecular Medicine
#8
of 18 outputs
Altmetric has tracked 22,790,780 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,551 research outputs from this source. They receive a mean Attention Score of 4.9. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 75,884 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.