| Title |
Cloning, expression, and pharmacological activity of BmK AS, an active peptide from scorpion Buthus martensii Karsch
|
|---|---|
| Published in |
Biotechnology Techniques, August 2007
|
| DOI | 10.1007/s10529-007-9499-y |
| Pubmed ID | |
| Authors |
Jian-Hua Shao, Yue-Qiu Wang, Xiao-Yan Wu, Rui Jiang, Rong Zhang, Chun-Fu Wu, Jing-Hai Zhang |
| Abstract |
BmK AS is a beta long-chain scorpion peptide from the venom of Buthus martensii Karsch (BmK). It was efficiently expressed as a soluble and functional peptide in Escherichia coli, and purified by metal chelating chromatography. About 4.2 mg/l purified recombinant BmK AS could be obtained. The recombinant BmK AS maintained a similar analgesic activity to the natural one in both the mouse-twisting test and hot-plate procedure. It also exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria. BmK AS is the first long-chain scorpion peptide reported to have antimicrobial activity, and is a valuable molecular scaffold for pharmacological research. |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 2 | 7% |
| Unknown | 26 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 36% |
| Researcher | 8 | 29% |
| Student > Master | 3 | 11% |
| Student > Bachelor | 1 | 4% |
| Other | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 4 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 46% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 11% |
| Medicine and Dentistry | 2 | 7% |
| Biochemistry, Genetics and Molecular Biology | 1 | 4% |
| Immunology and Microbiology | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 5 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2010.
All research outputs
#8,534,976
of 25,374,647 outputs
Outputs from Biotechnology Techniques
#827
of 2,762 outputs
Outputs of similar age
#28,352
of 78,843 outputs
Outputs of similar age from Biotechnology Techniques
#19
of 40 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,762 research outputs from this source. They receive a mean Attention Score of 3.9. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
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We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.