| Title |
Experimental in vivo measurements of light emission in plants: a perspective dedicated to David Walker
|
|---|---|
| Published in |
Photosynthesis Research, October 2012
|
| DOI | 10.1007/s11120-012-9780-3 |
| Pubmed ID | |
| Authors |
Hazem M. Kalaji, Vasilij Goltsev, Karolina Bosa, Suleyman I. Allakhverdiev, Reto J. Strasser, Govindjee |
| Abstract |
This review is dedicated to David Walker (1928-2012), a pioneer in the field of photosynthesis and chlorophyll fluorescence. We begin this review by presenting the history of light emission studies, from the ancient times. Light emission from plants is of several kinds: prompt fluorescence (PF), delayed fluorescence (DF), thermoluminescence, and phosphorescence. In this article, we focus on PF and DF. Chlorophyll a fluorescence measurements have been used for more than 80 years to study photosynthesis, particularly photosystem II (PSII) since 1961. This technique has become a regular trusted probe in agricultural and biological research. Many measured and calculated parameters are good biomarkers or indicators of plant tolerance to different abiotic and biotic stressors. This would never have been possible without the rapid development of new fluorometers. To date, most of these instruments are based mainly on two different operational principles for measuring variable chlorophyll a fluorescence: (1) a PF signal produced following a pulse-amplitude-modulated excitation and (2) a PF signal emitted during a strong continuous actinic excitation. In addition to fluorometers, other instruments have been developed to measure additional signals, such as DF, originating from PSII, and light-induced absorbance changes due to the photooxidation of P700, from PSI, measured as the absorption decrease (photobleaching) at about 705 nm, or increase at 820 nm. In this review, the technical and theoretical basis of newly developed instruments, allowing for simultaneous measurement of the PF and the DF as well as other parameters is discussed. Special emphasis has been given to a description of comparative measurements on PF and DF. However, DF has been discussed in greater details, since it is much less used and less known than PF, but has a great potential to provide useful qualitative new information on the back reactions of PSII electron transfer. A review concerning the history of fluorometers is also presented. |
Mendeley readers
The data shown below were compiled from readership statistics for 112 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Belgium | 1 | <1% |
| Unknown | 110 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 27 | 24% |
| Researcher | 18 | 16% |
| Student > Master | 11 | 10% |
| Student > Doctoral Student | 9 | 8% |
| Student > Bachelor | 5 | 4% |
| Other | 17 | 15% |
| Unknown | 25 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 38 | 34% |
| Biochemistry, Genetics and Molecular Biology | 12 | 11% |
| Environmental Science | 6 | 5% |
| Physics and Astronomy | 4 | 4% |
| Engineering | 4 | 4% |
| Other | 12 | 11% |
| Unknown | 36 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 January 2019.
All research outputs
#7,454,951
of 22,790,780 outputs
Outputs from Photosynthesis Research
#195
of 769 outputs
Outputs of similar age
#57,034
of 173,815 outputs
Outputs of similar age from Photosynthesis Research
#2
of 3 outputs
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