| Title |
Molecular cloning and expression of a novel klotho-related protein
|
|---|---|
| Published in |
Journal of Molecular Medicine, August 2000
|
| DOI | 10.1007/s001090000131 |
| Pubmed ID | |
| Authors |
Kensei Yahata, Kiyoshi Mori, Hiroshi Arai, Susumu Koide, Yoshihiro Ogawa, Masashi Mukoyama, Akira Sugawara, Shoichi Ozaki, Issei Tanaka, Yo-ichi Nabeshima, Kazuwa Nakao |
| Abstract |
Klotho protein is a novel beta-glucosidase-like protein produced predominantly in the kidney. The klotho mouse, which genetically lacks klotho gene expression, manifests various systemic phenotypes resembling aging. In the present study we succeeded in isolating a novel human protein structurally related to klotho protein. The protein possesses one beta-glucosidase-like domain and is 42% identical with klotho protein at the amino acid level. Unlike klotho protein, it possesses neither a signal sequence nor a transmembrane domain, suggesting that it is a cytosolic protein, and thus was termed cytosolic beta-glucosidase-like protein-1 (cBGL1). By Northern blot analysis cBGL1 mRNA was expressed most abundantly in the liver, followed by the small intestine, colon, spleen, and kidney. When klotho and cBGL1 gene expression was examined in renal cell carcinoma tissues, both klotho and cBGL1 mRNA levels in tumors were lower than those in nontumor regions, suggesting that renal epithelial cells may lose klotho and cBGL1 gene expression during the course of malignant transformation. In conclusion, we describe the primary structure and gene expression of a novel protein related to klotho protein. |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 47 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 23% |
| Researcher | 7 | 15% |
| Professor | 7 | 15% |
| Student > Master | 4 | 9% |
| Student > Bachelor | 3 | 6% |
| Other | 9 | 19% |
| Unknown | 6 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 26% |
| Biochemistry, Genetics and Molecular Biology | 10 | 21% |
| Medicine and Dentistry | 9 | 19% |
| Chemistry | 3 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Other | 4 | 9% |
| Unknown | 7 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2022.
All research outputs
#3,285,815
of 22,867,327 outputs
Outputs from Journal of Molecular Medicine
#122
of 1,551 outputs
Outputs of similar age
#3,215
of 37,650 outputs
Outputs of similar age from Journal of Molecular Medicine
#2
of 6 outputs
Altmetric has tracked 22,867,327 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,551 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done well, scoring higher than 88% of its peers.
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We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.