Title |
Evaluation of mRNA by Q-RTPCR and protein expression by AQUA of the M2 subunit of ribonucleotide reductase (RRM2) in human tumors
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Published in |
Cancer Chemotherapy and Pharmacology, October 2008
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DOI | 10.1007/s00280-008-0845-0 |
Pubmed ID | |
Authors |
Jill Kolesar, Wei Huang, Jens Eickhoff, Kristine Hahn, Dona Alberti, Steven Attia, William Schelman, Kyle Holen, Anne Traynor, Percy Ivy, George Wilding |
Abstract |
The purpose of this study was to evaluate baseline RRM2 protein and gene expression in tumors of patients receiving 3-AP. Tumor blocks from patients enrolled in phase I and II clinical studies using 3-AP, were evaluated for RRM2 gene and protein expression by quantitative real time polymerase chain reaction (Q-RTPCR) and automated quantitative analysis (AQUA). Esophageal and gastric cancers overexpressed RRM2 protein when compared to prostate cancer (Z-score, 0.68 +/- 0.94 SD, vs 0.41 +/- 0.84 SD, respectively; p = 0.04). Esophageal and gastric cancers also overexpressed RRM2 mRNA when compared to prostate cancer (relative gene expression 2.56 +/- 1.49 SD, vs 0.29 +/- 0.20 SD, respectively; p = 0.02). Protein and gene expression were moderately associated (Spearman's rank correlation = 0.30; p = 0.12). RRM2 gene and protein expression varies by tumor type. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 4% |
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Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 4 | 17% |
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Other | 1 | 4% |
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