| Title |
Monorhamnolipids and 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs) production using Escherichia coli as a heterologous host
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, June 2006
|
| DOI | 10.1007/s00253-006-0468-5 |
| Pubmed ID | |
| Authors |
Natividad Cabrera-Valladares, Anne-Pascale Richardson, Clarita Olvera, Luis Gerardo Treviño, Eric Déziel, François Lépine, Gloria Soberón-Chávez |
| Abstract |
Pseudomonas aeruginosa produces the biosurfactants rhamnolipids and 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs). In this study, we report the production of one family of rhamnolipids, specifically the monorhamnolipids, and of HAAs in a recombinant Escherichia coli strain expressing P. aeruginosa rhlAB operon. We found that the availability in E. coli of dTDP-L: -rhamnose, a substrate of RhlB, restricts the production of monorhamnolipids in E. coli. We present evidence showing that HAAs and the fatty acid dimer moiety of rhamnolipids are the product of RhlA enzymatic activity. Furthermore, we found that in the recombinant E. coli, these compounds have the same chain length of the fatty acid dimer moiety as those produced by P. aeruginosa. These data suggest that it is RhlAB specificity, and not the hydroxyfatty acid relative abundance in the bacterium, that determines the profile of the fatty acid moiety of rhamnolipids and HAAs. The rhamnolipids level produced in recombinant E. coli expressing rhlAB is lower than the P. aeruginosa level and much higher than those reported by others in E. coli, showing that this metabolic engineering strategy lead to an increased rhamnolipids production in this heterologous host. |
Mendeley readers
The data shown below were compiled from readership statistics for 112 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Malaysia | 1 | <1% |
| Spain | 1 | <1% |
| Mexico | 1 | <1% |
| Unknown | 108 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 21 | 19% |
| Student > Ph. D. Student | 20 | 18% |
| Student > Master | 19 | 17% |
| Student > Bachelor | 16 | 14% |
| Student > Doctoral Student | 6 | 5% |
| Other | 17 | 15% |
| Unknown | 13 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 37 | 33% |
| Biochemistry, Genetics and Molecular Biology | 27 | 24% |
| Environmental Science | 5 | 4% |
| Engineering | 5 | 4% |
| Chemical Engineering | 4 | 4% |
| Other | 14 | 13% |
| Unknown | 20 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2021.
All research outputs
#2,671,203
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#262
of 8,034 outputs
Outputs of similar age
#5,009
of 66,316 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#2
of 65 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 66,316 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 65 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.