| Title |
Amplification of plant genomic DNA by Phi29 DNA polymerase for use in physical mapping of the hypermethylated genomic region
|
|---|---|
| Published in |
Plant Cell Reports, May 2004
|
| DOI | 10.1007/s00299-004-0806-y |
| Pubmed ID | |
| Authors |
E. Adachi, K. Shimamura, S. Wakamatsu, H. Kodama |
| Abstract |
Plant genomes contain a heavily methylated region in which cytosines are methylated in both the symmetrical and asymmetrical sequences. The physical mapping of such a hypermethylated region is difficult because many restriction enzymes are sensitive to methylated cytosine residues in their recognition sites. The Phi29 DNA polymerase provides an efficient and representative amplification of the genomic DNA that is methylation-free. Using this amplified genomic DNA, we were able to show that a heavily methylated genomic DNA region becomes amenable to physical mapping with any restriction enzymes. This protocol will be especially useful for analysis of the heavily methylated region of plant genomes. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Bolivia, Plurinational State of | 1 | 8% |
| Netherlands | 1 | 8% |
| Unknown | 10 | 83% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 17% |
| Student > Ph. D. Student | 2 | 17% |
| Professor > Associate Professor | 2 | 17% |
| Researcher | 2 | 17% |
| Professor | 1 | 8% |
| Other | 2 | 17% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 75% |
| Biochemistry, Genetics and Molecular Biology | 1 | 8% |
| Unknown | 2 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 November 2010.
All research outputs
#7,454,951
of 22,790,780 outputs
Outputs from Plant Cell Reports
#771
of 2,182 outputs
Outputs of similar age
#18,660
of 57,753 outputs
Outputs of similar age from Plant Cell Reports
#5
of 13 outputs
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