| Title |
Identification of 48 homologues of phosphatidylethanol in blood by LC-ESI-MS/MS
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, February 2010
|
| DOI | 10.1007/s00216-010-3458-5 |
| Pubmed ID | |
| Authors |
H. Gnann, C. Engelmann, G. Skopp, M. Winkler, V. Auwärter, S. Dresen, N. Ferreirós, F. M. Wurst, W. Weinmann |
| Abstract |
Phosphatidylethanol (PEth) is an abnormal phospholipid carrying two fatty acid chains. It is only formed in the presence of ethanol via the action of phospholipase D (PLD). Its use as a biomarker for alcohol consumption is currently under investigation. Previous methods for the analysis of PEth included high-performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector (ELSD), which is unspecific for the different homologues--improved methods are now based on time of flight mass spectrometry (TOF-MS) and tandem mass spectrometry (MS/MS). The intention of this work was to identify as many homologues of PEth as possible. A screening procedure using multiple-reaction monitoring (MRM) for the identified homologues has subsequently been established. For our investigations, autopsy blood samples collected from heavy drinkers were used. Phosphatidylpropanol 16:0/18:1 (internal standard) was added to the blood samples prior to liquid-liquid extraction using borate buffer (pH 9), 2-propanol and n-hexane. After evaporation, the samples were redissolved in the mobile phase and injected into the LC-MS/MS system. Compounds were separated on a Luna Phenyl Hexyl column (50 mm x 2 mm, 3 microm) by gradient elution, using 2 mM ammonium acetate and methanol/acetone (95/5; v/v). A total of 48 homologues of PEth could be identified by using precursor ion and enhanced product ion scans (EPI). |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Netherlands | 1 | 1% |
| Austria | 1 | 1% |
| Brazil | 1 | 1% |
| Unknown | 63 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 15% |
| Student > Ph. D. Student | 9 | 13% |
| Student > Master | 5 | 7% |
| Student > Bachelor | 5 | 7% |
| Student > Doctoral Student | 4 | 6% |
| Other | 17 | 25% |
| Unknown | 17 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 13 | 19% |
| Medicine and Dentistry | 12 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 9% |
| Biochemistry, Genetics and Molecular Biology | 4 | 6% |
| Agricultural and Biological Sciences | 4 | 6% |
| Other | 9 | 13% |
| Unknown | 19 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 November 2016.
All research outputs
#3,798,945
of 25,374,917 outputs
Outputs from Analytical & Bioanalytical Chemistry
#457
of 9,619 outputs
Outputs of similar age
#19,204
of 172,638 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#7
of 52 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,619 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 172,638 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.