| Title |
Evaluation of combined growth media for in vitro cultivation of oropharyngeal biofilms on prosthetic silicone
|
|---|---|
| Published in |
Journal of Materials Science: Materials in Medicine, April 2018
|
| DOI | 10.1007/s10856-018-6051-7 |
| Pubmed ID | |
| Authors |
M. Leonhard, B. Zatorska, D. Moser, Y. Tan, B. Schneider-Stickler |
| Abstract |
In the upper aerodigestive tract, biofilm deposits by oropharyngeal microbes can cause failure of medical polymer devices like voice prostheses. Previous studies on testing of inhibitive strategies still lack of comparability due to varying study protocols concerning growth media, microbial species and growth conditions. Goal of the study was therefore to test cultivation of a mixed biofilm of isolated oropharyngeal microbes under in vitro growth conditions using mixtures of common growth media. Mixtures of yeast peptone dextrose medium (YPD), fetal bovine serum (FBS), RPMI 1640, Yeast nitrogen base medium (YNB) and brain heart infusion (BHI) were tested to grow mixed biofilm deposits of Candida albicans, Candida tropicalis, Staphylococcus aureus, Streptococcus epidermidis, Rothia dentocariosa and Lactobacillus gasseri on medical grade silicone. Periodic assessment of living biofilm was performed over 22 days by a digital microscope and the cultivated biofilm structures were analyzed by scanning electron microscopy after completion of the study. Mixtures of BHI, YPD and FBS improved microscopic growth of multispecies biofilm deposits over time, while addition of RPMI and YNB resulted in reduction of visible biofilm deposit sizes. A mixtures of FBS 30% + YPD 70% and BHI 30% + YPD 70% showed enhanced support of permanent surface growth on silicone. Growth kinetics of in vitro multispecies biofilms can be manipulated by using mixtures of common growth media. Using mixtures of growth media can improve growth of longterm multispecies oropharyngeal biofilm models used for in vitro testing of antibiofilm materials or coatings for voice prostheses. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 44 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 7 | 16% |
| Student > Ph. D. Student | 6 | 14% |
| Student > Master | 5 | 11% |
| Other | 4 | 9% |
| Student > Doctoral Student | 2 | 5% |
| Other | 4 | 9% |
| Unknown | 16 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 18% |
| Medicine and Dentistry | 5 | 11% |
| Immunology and Microbiology | 4 | 9% |
| Engineering | 3 | 7% |
| Agricultural and Biological Sciences | 3 | 7% |
| Other | 4 | 9% |
| Unknown | 17 | 39% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 April 2018.
All research outputs
#20,480,611
of 23,041,514 outputs
Outputs from Journal of Materials Science: Materials in Medicine
#1,294
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Outputs of similar age
#290,501
of 329,292 outputs
Outputs of similar age from Journal of Materials Science: Materials in Medicine
#12
of 14 outputs
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