| Title |
Quantitative determination of major phlorotannins in Ecklonia stolonifera
|
|---|---|
| Published in |
Archives of Pharmacal Research, April 2010
|
| DOI | 10.1007/s12272-010-0407-y |
| Pubmed ID | |
| Authors |
Hyun Ryul Goo, Jae Sue Choi, Dong Hee Na |
| Abstract |
Ecklonia stolonifera is a rich source of phlorotannins, which are responsible for the potent pharmacological effects associated with this seaweed. The purpose of this study was to develop a reversed-phase high-performance liquid chromatography method for the simultaneous determination of three major phlorotannins, eckol, dieckol, and phlorofucofuroeckol-A, in the extracts of Ecklonia stolonifera. The optimal chromatographic conditions were achieved on a Thermo Hypersil Gold C-18 column (250 x 4.6 mm i.d., 5 microm) using linear gradient elution of acetonitrile and water containing 0.1% formic acid at UV 254 nm. The separated phlorotannins were identified by liquid chromatography-mass spectrometry. The high-performance liquid chromatography method showed good linearity (r2 > 0.998), precision (1.4-9.5%), and accuracy (93.9-108.7%). The limits of detection ranged from 0.06 to 0.30 microg/mL and the lower limits of quantitation ranged from 0.2 to 1.0 microg/mL. Among phlorotannins, dieckol was the most abundant in both ethanol and ethyl acetate extracts of Ecklonia stolonifera. |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 3% |
| France | 1 | 3% |
| Unknown | 30 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 28% |
| Student > Ph. D. Student | 5 | 16% |
| Student > Master | 3 | 9% |
| Student > Bachelor | 2 | 6% |
| Lecturer | 2 | 6% |
| Other | 6 | 19% |
| Unknown | 5 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 28% |
| Medicine and Dentistry | 5 | 16% |
| Environmental Science | 4 | 13% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 9% |
| Chemistry | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 7 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 November 2013.
All research outputs
#7,454,951
of 22,790,780 outputs
Outputs from Archives of Pharmacal Research
#366
of 1,294 outputs
Outputs of similar age
#34,429
of 95,550 outputs
Outputs of similar age from Archives of Pharmacal Research
#4
of 22 outputs
Altmetric has tracked 22,790,780 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,294 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
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We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.