Title |
RNA-MATE: a recursive mapping strategy for high-throughput RNA-sequencing data
|
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Published in |
Bioinformatics, July 2009
|
DOI | 10.1093/bioinformatics/btp459 |
Pubmed ID | |
Authors |
Nicole Cloonan, Qinying Xu, Geoffrey J. Faulkner, Darrin F. Taylor, Dave T. P. Tang, Gabriel Kolle, Sean M. Grimmond |
Abstract |
Mapping of next-generation sequencing data derived from RNA samples (RNAseq) presents different genome mapping challenges than data derived from DNA. For example, tags that cross exon-junction boundaries will often not map to a reference genome, and the strand specificity of the data needs to be retained. Here we present RNA-MATE, a computational pipeline based on a recursive mapping strategy for placing strand specific RNAseq data onto a reference genome. Maximizing the mappable tags can provide significant savings in the cost of sequencing experiments. This pipeline provides an automatic and integrated way to align color-space sequencing data, collate this information and generate files for examining gene-expression data in a genomic context. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 8 | 4% |
Brazil | 4 | 2% |
Italy | 3 | 2% |
Australia | 3 | 2% |
Germany | 2 | 1% |
France | 2 | 1% |
Norway | 2 | 1% |
United Kingdom | 2 | 1% |
Sweden | 1 | <1% |
Other | 2 | 1% |
Unknown | 152 | 84% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 69 | 38% |
Student > Ph. D. Student | 35 | 19% |
Professor | 14 | 8% |
Student > Master | 13 | 7% |
Professor > Associate Professor | 12 | 7% |
Other | 26 | 14% |
Unknown | 12 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 110 | 61% |
Biochemistry, Genetics and Molecular Biology | 20 | 11% |
Computer Science | 14 | 8% |
Medicine and Dentistry | 13 | 7% |
Engineering | 3 | 2% |
Other | 7 | 4% |
Unknown | 14 | 8% |