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Mendeley readers
Attention Score in Context
| Title |
p53 and TFIIEα share a common binding site on the Tfb1/p62 subunit of TFIIH
|
|---|---|
| Published in |
Proceedings of the National Academy of Sciences of the United States of America, January 2008
|
| DOI | 10.1073/pnas.0707892105 |
| Pubmed ID | |
| Authors |
Paola Di Lello, Lisa M. Miller Jenkins, Caroline Mas, Chantal Langlois, Elena Malitskaya, Amélie Fradet-Turcotte, Jacques Archambault, Pascale Legault, James G. Omichinski |
| Abstract |
The general transcription factor IIH is recruited to the transcription preinitiation complex through an interaction between its p62/Tfb1 subunit and the alpha-subunit of the general transcription factor IIE (TFIIEalpha). We have determined that the acidic carboxyl terminus of TFIIEalpha (TFIIEalpha(336-439)) directly binds the amino-terminal PH domain of p62/Tfb1 with nanomolar affinity. NMR mapping and mutagenesis studies demonstrate that the TFIIEalpha binding site on p62/Tfb1 is identical to the binding site for the second transactivation domain of p53 (p53 TAD2). In addition, we demonstrate that TFIIEalpha(336-439) is capable of competing with p53 for a common binding site on p62/Tfb1 and that TFIIEalpha(336-439) and the diphosphorylated form (pS46/pT55) of p53 TAD2 have similar binding constants. NMR structural studies reveal that TFIIEalpha(336-439) contains a small domain (residues 395-433) folded in a novel betabetaalphaalphaalpha topology. NMR mapping studies demonstrate that two unstructured regions (residues 377-393 and residues 433-439) located on either side of the folded domain appear to be required for TFIIEalpha(336-439) binding to p62/Tfb1 and that these two unstructured regions are held close to each other in three-dimensional space by the novel structured domain. We also demonstrate that, like p53, TFIIEalpha(336-439) can activate transcription in vivo. These results point to an important interplay between the general transcription factor TFIIEalpha and the tumor suppressor protein p53 in regulating transcriptional activation that may be modulated by the phosphorylation status of p53. |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 2 | 6% |
| United States | 1 | 3% |
| Unknown | 32 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 34% |
| Researcher | 8 | 23% |
| Professor > Associate Professor | 5 | 14% |
| Student > Bachelor | 3 | 9% |
| Professor | 2 | 6% |
| Other | 4 | 11% |
| Unknown | 1 | 3% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 49% |
| Biochemistry, Genetics and Molecular Biology | 14 | 40% |
| Veterinary Science and Veterinary Medicine | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Medicine and Dentistry | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 1 | 3% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 August 2019.
All research outputs
#8,219,054
of 24,625,114 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#64,491
of 101,438 outputs
Outputs of similar age
#44,348
of 165,186 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#389
of 609 outputs
Altmetric has tracked 24,625,114 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 101,438 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 38.8. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 165,186 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 609 others from the same source and published within six weeks on either side of this one. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.