| Title |
Enhancement of Polyunsaturated Fatty Acid Production by Tn5 Transposon in Shewanella baltica
|
|---|---|
| Published in |
Biotechnology Techniques, June 2006
|
| DOI | 10.1007/s10529-006-9077-8 |
| Pubmed ID | |
| Authors |
Mitra Amiri-Jami, Haifeng Wang, Yukio Kakuda, Mansel W. Griffiths |
| Abstract |
Transposon Tn5 mutagenesis was used to generate random mutations in Shewanella baltica MAC1, a polyunsaturated fatty acid (PUFA)-producing bacterium. Three mutants produced 3-5 times more eicosapentaenoic acid (EPA 20:5 n-3) compared to the wild type at 10 degrees C. One of the mutants produced 0.3 mg EPA g(-1) when grown at high temperature (30 degrees C). Moreover, 2 mg docosahexaenoic acid (DHA 22:6 n-3) g(-1) was produced by S. baltica mutants at 4 degrees C. Sequencing of insertion mutation(s) showed 96% homology to trimethylamine N-oxide (TMAO) reductase gene and 85% homology to rRNA operons of E. coli. Tn5 transposon mutagenesis therefore is a suitable technique to increase PUFA formation in bacteria. |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 34% |
| Researcher | 7 | 22% |
| Professor > Associate Professor | 3 | 9% |
| Student > Master | 2 | 6% |
| Lecturer | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 8 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 41% |
| Biochemistry, Genetics and Molecular Biology | 6 | 19% |
| Environmental Science | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Social Sciences | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 9 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 February 2019.
All research outputs
#8,535,472
of 25,374,917 outputs
Outputs from Biotechnology Techniques
#827
of 2,762 outputs
Outputs of similar age
#30,133
of 88,125 outputs
Outputs of similar age from Biotechnology Techniques
#20
of 46 outputs
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