| Title |
Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansiinfections
|
|---|---|
| Published in |
Kinetoplastid Biology and Disease, September 2004
|
| DOI | 10.1186/1475-9292-3-3 |
| Pubmed ID | |
| Authors |
Filip Claes, Magda Radwanska, Toyo Urakawa, Phelix AO Majiwa, Bruno Goddeeris, Philip Büscher |
| Abstract |
BACKGROUND: Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2 based Polymerase Chain Reaction (PCR). RESULTS: This PCR yielded a 205 bp product in all T. evansi and in seven out of nine T. equiperdum strains tested. This product was not detected in the DNA from T. b. brucei, T. b. gambiense, T. b. rhodesiense, T. congolense, T. vivax and T. theileri parasites. The Rotat 1.2 PCR detects as few as 10 trypanosomes per reaction with purified DNA from blood samples, i.e. 50 trypanosomes/ml. CONCLUSION: PCR amplification of the RoTat 1.2 VSG gene is a specific marker for T. evansi strains, except T. evansi type B, and is especially useful in dyskinetoplastic strains where kDNA based markers may fail to amplify. Furthermore, our data support previous suggestions that some T. evansi stocks have been previously misclassified as T. equiperdum. |
Mendeley readers
The data shown below were compiled from readership statistics for 85 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Ecuador | 1 | 1% |
| France | 1 | 1% |
| Unknown | 83 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 16 | 19% |
| Student > Ph. D. Student | 12 | 14% |
| Researcher | 9 | 11% |
| Student > Doctoral Student | 8 | 9% |
| Student > Bachelor | 7 | 8% |
| Other | 18 | 21% |
| Unknown | 15 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 27% |
| Veterinary Science and Veterinary Medicine | 18 | 21% |
| Biochemistry, Genetics and Molecular Biology | 11 | 13% |
| Medicine and Dentistry | 5 | 6% |
| Immunology and Microbiology | 4 | 5% |
| Other | 7 | 8% |
| Unknown | 17 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2013.
All research outputs
#8,534,976
of 25,374,647 outputs
Outputs from Kinetoplastid Biology and Disease
#2
of 9 outputs
Outputs of similar age
#23,998
of 73,094 outputs
Outputs of similar age from Kinetoplastid Biology and Disease
#1
of 1 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one scored the same or higher as 7 of them.
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We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them