| Title |
A novel beta-propeller phytase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, January 2009
|
| DOI | 10.1007/s00253-008-1835-1 |
| Pubmed ID | |
| Authors |
Huoqing Huang, Na Shao, Yaru Wang, Huiying Luo, Peilong Yang, Zhigang Zhou, Zhichun Zhan, Bin Yao |
| Abstract |
A phytase with high activity at neutral pH and typical water temperatures ( approximately 25 degrees C) could effectively hydrolyze phytate in aquaculture. In this study, a phytase-producing strain, Pedobacter nyackensis MJ11 CGMCC 2503, was isolated from glacier soil, and the relevant gene, PhyP, was cloned using degenerate PCR and thermal asymmetric interlaced PCR. To our knowledge, this is the first report of detection of phytase activity and cloning of phytase gene from Pedobacter. PhyP belongs to beta-propeller phytase family and shares very low identity ( approximately 28.5%) with Bacillus subtilis phytase. The purified recombinant enzyme (r-PhyP) from Escherichia coli displayed high specific activity for sodium phytate of 24.4 U mg(-1). The optimum pH was 7.0, and the optimum temperature was 45 degrees C. The K (m), V (max), and k (cat) values were 1.28 mM, 71.9 micromol min(-1) mg(-1), and 45.1 s(-1), respectively. Compared with Bacillus phytases, r-PhyP had higher relative activity at 25 degrees C (r-PhyP (>50%), B. subtilis phytase (<8%)) and hydrolyzed phytate from soybean with greater efficacy at neutral pH. These characteristics suggest that r-PhyP might be a good candidate for an aquatic feed additive in the aquaculture industry. |
Mendeley readers
The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 2% |
| Chile | 1 | 2% |
| Unknown | 49 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 18% |
| Student > Ph. D. Student | 6 | 12% |
| Professor | 5 | 10% |
| Student > Bachelor | 4 | 8% |
| Student > Doctoral Student | 4 | 8% |
| Other | 13 | 25% |
| Unknown | 10 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 45% |
| Biochemistry, Genetics and Molecular Biology | 10 | 20% |
| Chemistry | 2 | 4% |
| Immunology and Microbiology | 2 | 4% |
| Veterinary Science and Veterinary Medicine | 1 | 2% |
| Other | 3 | 6% |
| Unknown | 10 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 April 2018.
All research outputs
#5,089,278
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#1,221
of 8,034 outputs
Outputs of similar age
#27,926
of 177,107 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#9
of 36 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 177,107 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 78% of its contemporaries.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.